Sequences from confirmation amplicon


Confirmation sequences for line 468C04 and hit At4g31700 are shown.
Line ID 468C04
Locus At4g31700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15346992
Primers used for PCR 30kh - 8474, show primer details
   
Sequence Name 81-K113669-0022-468-C04C-30kh
GenBank Accession LN514095 [GenBank]
Sequencing primer gene specific primer, 30kh: 5'-TTTTGCTTCTCTGTTTCATCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K113669-0022-468-C04C-30kh
GATCTATTGATTGGTGTTTGTATATATCACTGTTAAATTGATAGGTCTAGTAAACAGTAA
TTGTCAATTTTTCTATGTTCGTCTTATAAGCTTAATAAGAGTTAGAATCTTCTTCTATGT
TTAATACTTACATTAAAATAAGTTTCGTATCTGCTCATAAACATATCTGGATTATATTAT
TCAGCTTGATTTATGTTGATGCAGACGTGCGTTTTACGACAAGAGAATCTCTCAAGAAGT
CAGTGGAGATGCTTTGGGCGAGGTACAGATTCCATTTTTCTTACTTTGATCTAATGAGTG
TGAAGATTAGCATTTCGAAGCAGATGATGATGTTTTGTTTATGAATTGTTGTTATTGCAG
GAGTTCAAAGGATACGTTTTCAAGATCAAGGGTGGTTGCGATAAGCAAGGTTTCCCAATG
AAGCAGGGAGTTTTGACTCCAGGCCGTGTTCGCCTTTTGCTTCACCGAGGTAACTAAACA
TAATACCTTTTCTTACACTTTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 50-K113666-0022-468-C04C-8409
GenBank Accession LN507235 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K113666-0022-468-C04C-8409
TTACATTGAATATATCCTGCAAAGTGTAAGAAAAGGTATTATGTTTAGTTACCTCGGTGA
AGCAAAAGGCGAACACGGCCTGGAGTCAAAACTCCCTGCTTCATTGGGAAACCTTGCTTA
TCGCAACCACCCTTGATCTTGAAAACGTATCCTTTGAACTCCTGCAATAACAACAATTCA
TAAACAAAACATCATCATCTGCTTCGAAATGCTAATCTTCACACTCATTAGATCAAAGTA
AGAAAAATGGAATCTGTACCTCGCCCAAAGCATCTCCACTGACTTCTTGAGAGATTCTCT
TGTCGTAAAACGCACGTCTGCATCAACATAAATCAAGCTGAATAATATAATCCAGATATG
TTTATGAGCAGATACGAAACTTATTTTAATGTAAGTATTAAACATAGAAGAAGATTCTAA
CTCTTATTAAGCTTATAAGACGAACATAGAAAAATTGACAATTACTGTTTACTAGACCTA
TCAATTTAACAGTGATATATACAAACACCAATCAATAGATCTGGCAACCTAGCACGAAAT
ATCTATTAAAAATGAATGAAGATGAAACAGAGAAGCAAA

Last Updated on 06.12.2018 16:11