Sequences from confirmation amplicon


Confirmation sequences for line 370D04 and hit At4g31715 are shown.
Line ID 370D04
Locus At4g31715 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15353789
Primers used for PCR 54zg - 8474, show primer details
   
Sequence Name 77-K126522-0022-370-D04C-54zg
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 54zg: 5'-AGAATATTTTGCTGATAGCCCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K126522-0022-370-D04C-54zg
GGAATTCCTCAAAATCTTTGCCTAGTAAGGACGGTCATGTGTCTCAGATCCCTGATTCTC
TGTTGTGTTTCAGTTAAAAACAAAACTCACTTGTCTTGCTAGTCCTCAATTACTACATTA
CATGTATATTCGTTTGTTGTTTGTTCTCTGGTGATTTTTGGGGTTCTACATCACTTATCC
ACCATTCTTGCAAGTCTCCAAGTCGTGAAAATAAGATAAGTATCCATCCTTGTGATTAAC
TGGATACTGTAGGATTGAAAAACCACAAAGTATATTTTCCATCTAGCAATGATGAACCTA
AGTGCTTCTGATCTAACTTGAAACATTGCCACACTCTGTTGTATACCTTTAGACAATCCC
CCTTTGTAACCGCGGCATCTCAATAATAAAGTTACTTTTTGGAATTTAGCAATTGAAAAA
AGCTGTTCGAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATATAGATGTCACGCAGCGTTA
   
Sequence Name 69-K126521-0022-370-D04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K126521-0022-370-D04C-8409
TTGAAATTCGAACAGCTTTTTTCATTGCTAAATTCCAAAAAGTAACTTTATTATTGAGAT
GCCGCGGTTACAAAGGGGGATTGTCTAAAGGTATACAACAGAGTGTGGCAATGTTTCAAG
TTAGATCAGAAGCACTTAGGTTCATCATTGCTAGATGGAAAATATACTTTGTGGTTTTTC
AATCCTACAGTATCCAGTTAATCACAAGGATGGATACTTATCTTATTTTCACGACTTGGA
GACTTGCAAGAATGGTGGATAAGTGATGTAGAACCCCAAAAATCACCAGAGAACAAACAA
CAAACGAATATACATGTAATGTAGTAATTGAGGACTAGCAAGACAAGTGAGTTTTGTTTT
TAACTGAAACACAACAGAGAATCAGGGATCTGAGACACATGACCGTCCTTACTAGGCAAA
GATTTTGAGGAATTCCTGGTTTCTATTCGTCCCTTGTTGCAGGGTCCATTCCAGTCGAAG
GGCTATCAGCAAAATATTCT

Last Updated on 06.12.2018 16:11