Sequences from confirmation amplicon


Confirmation sequences for line 183B09 and hit At4g31970 are shown.
Line ID 183B09
Locus At4g31970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15464100
Primers used for PCR MS61 - 8474, show primer details
   
Sequence Name 31-K017177-022-183-B09C-MS61
GenBank Accession LN499943 [GenBank]
Sequencing primer gene specific primer, MS61: 5'-CTAGCATTAAATCTACCTGCCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K017177-022-183-B09C-MS61
TAACTACACAAATTTTGATGTTTTTTAGATGAACTAGATATATAAACCTAACCAAATTAA
CTGGGATTTTGTCCAAAAATTATTGTTTTCTAAAATACAAAGTTAGTTATATAAACAAGT
TTAGGTGGAAAGACGTAAATGGATAACATGTTTATTTTGTAAAAAATTACTTCCACCTTA
AATTGATGG
   
Sequence Name 33-K017177-022-183-B09C-8409
GenBank Accession LN500789 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K017177-022-183-B09C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTGATAAACCTCTCTGG
TCGAAATTCGTTTGGCTCCATATAAACCCTCGGATCTCTTTGGATTTTCCATACATTCAC
TAACATTCTTGTGCCGCGACGAACGTTGTAACCAGCGACCGTGCAATCTTCTATCGCCTC
TCGATGGCCTAAGAGAGGACCAGCTGGATACAATCTCAATGTTTCTTTGATAATCGCTTG
AATATACACCAGATTTTCTATGTCTGAATCCTCGACGTTCCTGTCTCTGCCGACGTGGAT
GTCGATCTCATCTTGTGCTTTCTTTAACATGTCCTTATTGTTTAGAAGAAGAGAAATGGC
CCATGTAAGGGTTGATGGTGAAGTCTCACTTCCTCCAAGAATCAGTGCCTGTTATTCAAT
TTATTATCATTATATGACATACATTAAAACAATATACTTATAAGTATCATCTAATCAGGA
TTCGCCTAATCCCATCAATTTAAGGTTGAAGTAATTTCTTACAAAATAAACATGTTATCA
TTTACGTCTTTCCACCTAAACTTGTTTATATAACTAACTTTGTATTTTAGAAAACAATAA
TTTTTGGACAAAATCCCAGTTAATTTGGTTAGGTTTATATATCTAGTTCATCTAAAAAAC
ATCAAAATTTGTGTAGTTAAAGATGTACCAGGCAGG

Last Updated on 06.12.2018 16:11