Sequences from confirmation amplicon


Confirmation sequences for line 243E09 and hit At4g32150 are shown.
Line ID 243E09
Locus At4g32150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15527022
Primers used for PCR KS51 - 8474, show primer details
   
Sequence Name 62-K033043-022-243-E09C-8409
GenBank Accession LN510677 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K033043-022-243-E09C-8409
TTGAATAATACAGTTAGGATCATTAGAGTAATAGTCAATCTGCTGACTGAGAACTCTAGA
GAATTCCTCATTCATTGCATAAGCTAGTGCTGTATGAACAGCCCTGCCATAAGTCCGTAC
GAATCTCTGGTGAATATCCTCCAAAAAGGCAAAAGGAATTCTCCCTGACATAAAAAAGTT
TTGACTTTTGAGTTAGAAAGACATTCACATCTCTCTAATGCACAGCTTCTCGGATATAAT
TGTGTTGATGTTATGTCCAAAAAAATCAAATTCATTCACATCACAATATAAGACCAAAGG
TCTCGAATTC
   
Sequence Name 30-K033043-022-243-E09C-KS51
GenBank Accession LN499616 [GenBank]
Sequencing primer gene specific primer, KS51: 5'-TGAATTCGAGACCTTTGGTCTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K033043-022-243-E09C-KS51
GATTGATTTTTTGGAATAACATCAACACAATTATATCCGAGAAGCTGTGCATTAGAGAGA
TGTGAATGTCTTTCTAACTCAAAAGTCAAAACTTTTTTATGTCAGGGAGAATTCCTTTTG
CCTTTTTGGAGGATATTCACCAGAGATTCGTACGGACTTATGGCAGGGCTGTTCATACAG
CACTAGCTTATGCAATGAATGAGGAATTCTCTAGAGTTCTCAGTCAGCAGATTGACTATT
ACTCTAATGATCCTAACTGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCNAAATGG

Last Updated on 06.12.2018 16:11