Sequences from confirmation amplicon


Confirmation sequences for line 574G12 and hit At4g32150 are shown.
Line ID 574G12
Locus At4g32150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15527382
Primers used for PCR MZ95 - 8474, show primer details
   
Sequence Name 87-K027351-022-574-G12C-8409
GenBank Accession LN514705 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K027351-022-574-G12C-8409
CACTTTGGTGACGTGGAATTCAAAGTCTAATCCTTTTTTGCTCTGAACGATCGCTCTGTT
ATTGAATTCGAGACCTTTGGTCTTATATTGTGATGTGAATGAATTTGATTTTTTTGGACA
TAACATCAACACAATTATATCCGAGAAGCTGTGCATTAGAGAGATGTGAATGTCTTTCTA
ACTCAAAAGTCAAAACTTTTTTATGTCAGGGAGAATTCCTTTTGCCTTTTTGGAGGATAT
TCACCAGAGATTCGTACGGACTTATGGCAGGGCTGTTCATACAGCACTAGCTTATGCAAT
GAATGAGGAATTCTCTAGAGTTCTCAGTCAGCAGATTGACTATTACTCTAATGATCCTAA
TGCCGATAGGATTAATCGTATTAAGGGTGAAATGAATCAGGTTTGATGAGTTTGTCTTAC
AAGTTCAGTCGGATTGTGAAGTTGTTTAGAGACATGGTTCTGAGTCTTGGCTTCACTGTT
TGACAATACATCC
   
Sequence Name 88-K027351-022-574-G12C-MZ95
GenBank Accession LN514810 [GenBank]
Sequencing primer gene specific primer, MZ95: 5'-AAAACCAAAAAGAAAAACCAGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K027351-022-574-G12C-MZ95
TGTCTCTAACACTTCACAATCCGACTGAACTTGTAAGACAAACTCATCAAACCTGATTCA
TTTCACCCTTAATACGATTAATCCTATCGGCATTAGGATCATTAGAGTAATAGTCAATCT
GCTGACTGAGAACTCTAGAGAATTCCTCATTCATTGCATAAGCTAGTGCTGTATGAACAG
CCCTGCCATAAGTCCGTACGAATCTCTGGTGAATATCCTCCAAAAAGGCAAAAGGAATTC
TCCCTGACATAAAAAAGTTTTGACTTTTGAGTTAGAAAGACATTCACATCTCTCTAATGC
ACAGCTTCTCGGATATAATTGTGTTGATGTTATGTCCAAAAAAATCAAATTCATTCACAT
CACAATATAAGACCAAAGGTCTCGAATTCAATAACAGAGCGATCGTTCAGAGCAAAAAAG
GATTAGACTTTTGAATTCCACTGTCACCAAAGTTGGAAACTTCCTCAAAATTAGCAAAGT
ATGCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11