Sequences from confirmation amplicon


Confirmation sequences for line 751E11 and hit At4g32150 are shown.
Line ID 751E11
Locus At4g32150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15526138
Primers used for PCR 23an - 8474, show primer details
   
Sequence Name 71-K107287-0022-751-E11C-23an
GenBank Accession LN512571 [GenBank]
Sequencing primer gene specific primer, 23an: 5'-GTGATGTAGCCGTCTTTTCTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K107287-0022-751-E11C-23an
TACCGTAAGTGACGCTTTTATTTTTTTTGGTTCACCAAACATTTTAGTATTTTGATCATA
TATGGATATAAATTCCATCCTTTTAGTTAGTAAACATGGTGTTGATAAAAACAAAATTTT
ATATTTGAAATGCTGCTATTATATAAATAAAAGGTTAAATCCACCTACTATAATTTATTT
AGCTATTATTAGGTGGCATGACAAGATGTCAAGTTGGTGAATGAGGTGCGTCTGGCCTTA
ACTAGAGATGATACCCATATTTTATTTTCGGACGACAATTTATGACACTTGTTGTATCTT
GTTCTCCTATCTTTCTTTTCTAATATTTACATTCGATTTATGATCAACAAATGGCTTCAA
TTGTAAAATGACAAGAATTTGTAGGGTCAACGACGTACAAGATCATTCAACTGCCGACTT
CAGAAGTTGGTGTGGATATACGAGAGAGGAAGTCACATAAACAAAGAACATGAAGATTCA
AAGAGTTGTATGAACATGATGTCAACAAAGATGGTCAATATGGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 55-K107286-0022-751-E11C-8409
GenBank Accession LN508831 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K107286-0022-751-E11C-8409
CGGACTGAGCCATATTGACATCTTTGTTGACATCATGTTCATACAACTCTTTGAATCTTC
ATGTTCTTTGTTTATGTGACTTCCTCTCTCGTATATCCACACCAACTTCTGAAGTCGGCA
GTTGAATGATCTTGTACGTCGTTGACCCTACAAATTCTTGTCATTTTACAATTGAAGCCA
TTTGTTGATCATAAATCGAATGTAAATATTAGAAAAGAAAGATAGGAGAACAAGATACAA
CAAGTGTCATAAATTGTCGTCCGAAAATAAAATATGGGTATCATCTCTAGTTAAGGCCAG
ACGCACCTCATTCACCAACTTGACATCTTGTCATGCCACCTAATAATAGCTAAATAAATT
ATAGTAGGTGGATTTAACCTTTTATTTATATAATAGCAGCATTTCAAATATAAAATTTTG
TTTTTATCAACACCATGTTTACTAACTAAAAGGATGGAATTTATATCCATATATGATCAA
AATACTAAAATGTTTGGTTGAACCAAAAAAAATAAAAAGCGTCGACTTTACGGTTTAAAT
ATCGTTACAGAAAAAGAAGAAAAGAAAAGACGGCTACATCA

Last Updated on 06.12.2018 16:11