Sequences from confirmation amplicon


Confirmation sequences for line 397H06 and hit At4g34690 are shown.
Line ID 397H06
Locus At4g34690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16556105
Primers used for PCR KP49 - 8474, show primer details
   
Sequence Name 59-K034068-022-397-H06C-KP49
GenBank Accession LN509917 [GenBank]
Sequencing primer gene specific primer, KP49: 5'-TGCAACCTTTGTAATCTCATCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K034068-022-397-H06C-KP49
TGGCCTCATCTCTTTCTTAACTCATGGATAACCACACTCTTCTTGTTTATCTCCAACCAC
AAGTCGGCTAGGCTTCTATCCGTTTTAGAGGTGTTCCATAACCCCCAAACGAAGGAGATG
CAAGTAAGAGAGCCAAAGAAACACCAAGTGAGAAACGAGGTATAGGTTTGTGAGAAATCT
CTGGAGGCCTTACCTAGGCAAGTGTACTCCACAAAAGTTTGATGCTCCTTCCCTTTAGCT
TTAGCCAGTAGTTGAAGCTTCTTAGCTTGAGATATGTTGATAAATTTGAGAAACTTCCAT
TTGCGTTTGACGCTCCTCTCGCATGATCTCTTGACCCCGATGTAATGAAGCCTAGTTGCT
AAAAGTTTAAAAACCTTGAACCATGTCGATTGTGGATCGTCATTAGGTGTGTAATCATCA
TCGTCATTATTGCTGGATCCATCATCACTCTTTTTCCCATCGCCGGATCCATCATTGCTC
TTTTTCTCACCGGATCCATCATCATCACTCTTTTTTTCACCAGATCCATCATTAGAATAA
ATTAAAAAAATTAAAAAATTTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTACATTCACGTCCAAATGGG
   
Sequence Name 43-K034068-022-397-H06C-8409
GenBank Accession LN504752 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K034068-022-397-H06C-8409
AAGCCATTTAAATTTTTTAATTTTTTTAATTTATTCTAATGATGGATCTGGTGAAAAAAA
GAGTGATGATGATGGATCCGGTGAGAAAAAGAGCAATGATGGATCCGGCGATGGGAAAAA
GAGTGATGATGGATCCAGCAATAATGACGATGATGATTACACACCTAATGACGATCCACA
ATCGACATGGTTCAAGGTTTTTAAACTTTTAGCAACTAGGCTTCATTACATCGGGGTCAA
GAGATCATGCGAGAGGAGCGTCAAACGCAAATGGAAGTTTCTCAAATTTATCAACATATC
TCAAGCTAAGAAGCTTCAACTACTGGCTAAAGCTAAAGGGAAGGAGCATCAAACTTTTGT
GGAGTACACTTGCCTAGGTAAGGCCTCCAGAGATTTCTCACAAACCTATACCTCGTTTCT
CACTTGGTGTTTCTTTGGCTCTCTTACTTGCATCTCCTTCGTTTGGGGGTTATGGAACAC
CTCTAAAACGGATAGAAGCCTAGCCGACTTGTGGTTGGAGATAAACAAGAAGAGTGTGGT
TATCCATGAGTTTAAGAAAGAGATGAGTGCCAAAAATGCTCTCTACATGCACCGGCTTGA
TGAGATT

Last Updated on 06.12.2018 16:11