Sequences from confirmation amplicon


Confirmation sequences for line 365G08 and hit At4g34960 are shown.
Line ID 365G08
Locus At4g34960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16650000
Primers used for PCR 22db - 8474, show primer details
   
Sequence Name 28-K109642-0022-365-G08C-22db
GenBank Accession LN498887 [GenBank]
Sequencing primer gene specific primer, 22db: 5'-GTGTTCTAGGGATCACTTCTTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K109642-0022-365-G08C-22db
ATTTCGCTTTCTGTGATTGAATCTGCAGGCAGGATTGTTATCGGACTATATGGCACTGTT
GTACCCAAAACTGTAGGTATGTCTTTGGCATCTTGAATGCAAATCTCGGGATTTGGTTAA
TTGTTTTAAATAGTTTCTAATGTAAGATTTCTTTACTTTTTGCAGAAAATTTCAGGGCTT
TATGCACAGGGGAGAAGGGTAAAACCTCAAGCGGAAAGCCTTTACATTATAAGGGAACAC
CATTTCATCGTATAATATCTGGATTTGTAATCCAAGGAGGAGACATTATCCATGGTGATG
GAAAAAGTAGTGACTCAATCTATGGTGGAACCTTTCCTGACGAAAATTTCAAGATACAGC
ATTCACACGCAGGTGCGCATATACATACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTA
   
Sequence Name 12-K109633-0022-365-G08C-8409
GenBank Accession LN491621 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K109633-0022-365-G08C-8409
TGCTGTTCTTGAATTTTCGTCAGGAAAGGTTCCACCATAGATTGAGTCACTACTTTTTCC
ATCACCATGGATAATGTCTCCTCCTTGGATTACAAATCCAGATATTATACGATGAAATGG
TGTTCCCTTATAATGTAAAGGCTTTCCGCTTGAGGTTTTACCCTTCTCCCCTGTGCATAA
AGCCCTGAAATTTTCTGCAAAAAGTAAAGAAATCTTACATTAGAAACTATTTAAAACAAT
TAACCAAATCCCGAGATTTGCATTCAAGATGCCAAAGACATACCTACAGTTTTGGGTACA
ACAGTGCCATATAGTCCGATAACAATCCTGCCTGCAGATTTCAATCACAGAAAGCGAAAT
CAACAGAAGTTGATGGAAGCTAATGGCAAAGAAGTGATCCCTAGAACA

Last Updated on 06.12.2018 16:11