Sequences from confirmation amplicon


Confirmation sequences for line 763E09 and hit At4g35920 are shown.
Line ID 763E09
Locus At4g35920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17012317
Primers used for PCR BK79 - 8474, show primer details
   
Sequence Name 32-K105013-0022-763-E09C-BK79
GenBank Accession LN500577 [GenBank]
Sequencing primer gene specific primer, BK79: 5'-TCTGCAAAGTTCTCAAAGATTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K105013-0022-763-E09C-BK79
TCTTTTTCCGTGTGGTACTTTGGCAAAGATTGCCACTGCAGCATCCAACAGGCACATCTG
TAAGGGATATTATTGAATACCGTGACTTTATTAATTTTCAATCTTTTAGTAATACATGTG
AAAGCTAATAAGTCAACTGGATTTTTTTTAATTTTCGCTCTCCCAGCTTCAGCTGAAGCA
TGTAATGAGCTAATGGCGTATTCTTTGATACTTTCTTGTTGCTGCTATACTTGTTGCGTG
AGGAGGAAACTCCGGAAGACACTGAACATCACGGTATTACATTCTCATTCTCTTCCACTC
GCTTGAATCCTTACCATTAATCCCATTTGATCCCAAAAATCTATATGCTTCCGAGAAAAT
TTCTATCATTTGCATACCATAAACAACAAATGAAATTTCGGTTAAAGATTAAGTAGACAC
CTGCAAAAAAATTCAGTAGTAACTATCATGGTTGAGATTGAAATGGCTATGTTTATGCCA
CTCAATGTAGAGTCGGAATGAATGCTTAATGTTTTCTGATTTCGCAGGGAGGATTCATTG
ACGATTCATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 16-K105014-0022-763-E09C-8409
GenBank Accession LN493424 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K105014-0022-763-E09C-8409
CATTTACAATTGAATATATCCTGCATGAATCGTCAATGAATCCTCCCTGCGAAATCAGAA
AACATTAAGCATTCATTCCGACTCTACATTGAGTGGCATAAACATAGCCATTTCAATCTC
AACCATGATAGTTACTACTGAATTTTTTTGCAGGTGTCTACTTAATCTTTAACCGAAATT
TCATTTGTTGTTTATGGTATGCAAATGATAGAAATTTTCTCGGAAGCATATAGATTTTTG
GGATCAAATGGGATTAATGGTAAGGATTCAAGCGAGTGGAAGAGAATGAGAATGTAATAC
CGTGATGTTCAGTGTCTTCCGGAGTTTCCTCCTCACGCAACAAGTATAGCAGCAACAAGA
AAGTATCAAAGAATACGCCATTAGCTCATTACATGCTTCAGCTGAAGCTGGGAGAGCGAA
AATTAAAAAAAATCCAGTTGACTTATTAGCTTTCACATGTATTACTAAAAGATTGAAAAT
TAATAAAGTCACGGTATTCAATAATATCCCTTACAGATGTGCCTGTTGGATGCTGCAGTG
GCAATCTTTGCCAAAGTACCACACGGGAAAAAGAATGTCTTGAAGCCTGCGGTTCCATCA
TTGAATCTTTGA

Last Updated on 06.12.2018 16:11