Sequences from confirmation amplicon


Confirmation sequences for line 338B01 and hit At4g37685 are shown.
Line ID 338B01
Locus At4g37685 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17705629
Primers used for PCR 06aj - 8474, show primer details
   
Sequence Name 10-K106680-0022-338-B01C-8409
GenBank Accession LN490671 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K106680-0022-338-B01C-8409
TTTACAGTGTTTTTTGGGAATTATTATGTTTTTTAGGGCCATATGCATATTGAATTATTA
TTCAGCCATGTTTTTTAGGGCTGATTCAGTGGGATTCTTCGAAGAAGAAAATGGAAGAGA
CAAGTGCAAGTGCTGCATGAACTGTCTCAAGGCTACTCACTTCAATAATGTGAAAGTGTC
ATGTATCTAGTTTGATAAACGACACATCTCAATGCTGGTGGCAGTGGCTGAAAAGAAGCT
CTAGAAGGAAGCAAATGCAAGAAATTTAAACTGCATTTAGAAGACAGTAACTAAGGAGTT
TGAGTTAGCTGTCTGCATCATGAGAAGATTACATAAGTAACTACTCAAGGGAAATTCACT
TGCAGGTTAGTCAAGTGAACGTTTTTTCAAAGTATCAGAGAATCATAAAGAAGACAAAGG
CTCTAGTTTAGNTCTGGA
   
Sequence Name 26-K106681-0022-338-B01C-06aj
GenBank Accession LN497944 [GenBank]
Sequencing primer gene specific primer, 06aj: 5'-TTCCAGACTCAAACTAGAGCCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K106681-0022-338-B01C-06aj
CGTTCACTTGACTAACCTGCAAGTGAATTTCCCTTGAGTAGTTACTTATGTAATCTTCTC
ATGATGCAGACAGCTAACTCAAACTCCTTAGTTACTGTCTTCTAAATGCAGTTTAAATTT
CTTGCATTTGCTTCCTTCTAGAGCTTCTTTTCAGCCACTGCCACCAGCATTGAGATGTGT
CGTTTATCAAACTAGATACATGACACTTTCACATTATTGAAGTGAGTAGCCTTGAGACAG
TTCATGCAGCACTTGCACTTGTCTCTTCCATTTTCTTCTTCGAAGAATCCCACTGAATCA
GCCCTAAAAAACATGGCTGAATAATAATTCAATATGCATATGGCCCTAAAAAACATAATA
ATTCCCAAAAAACACTTGTAAATGACTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11