Sequences from confirmation amplicon


Confirmation sequences for line 804G12 and hit At4g38900 are shown.
Line ID 804G12
Locus At4g38900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18141112
Primers used for PCR 36lx - 8474, show primer details
   
Sequence Name 33-K114217-0022-804-G12C-36lx
GenBank Accession LN501083 [GenBank]
Sequencing primer gene specific primer, 36lx: 5'-ACTTCTTCTTCCATTCCCAAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K114217-0022-804-G12C-36lx
CCGCTCATCAGCTCCTCAATTCTCCAAGCCTTTCAGTGACAGTGGCAAACGAATCGGTGT
TCCTCCGTCGCACCCCAACTTAATCCCACCGACTTCTCCGTTTTCTCAGATCCCGACCAC
CCGACAACCCGGTTCGCATAATTTTAACCCGGGAGGAGCTAATCATTCACGGTCAATGTC
ACAGCCCAACTCTTTCTTCTCTTTTGACTCCTTACCTCCGTTAAGCCCTTCTCCGTTTCG
AGATCACGATGTTTCAATGGAGGATAGAGATTCCGGCGTGTTTAACAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 15-K114216-0022-804-G12C-8409
GenBank Accession LN493065 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114216-0022-804-G12C-8409
TTGTTAACACGCCGGAATCTCTATCCTCCATTGAAACATCGTGATCTCGAAACGGAGAAG
GGCTTAACGGAGGTAAGGAGTCAAAAGAGAAGAAAGAGTTGGGCTGTGACATTGACCGTG
AATGATTAGCTCCTCCCGGGTTAAAATTATGCGAACCGGGTTGTCGGGTGGTCGGGATCT
GAGAAAACGGAGAAGTCGGTGGGATTAAGTTGGGGTGCGACGGAGGAACACCGATTCGTT
TGCCACTGTCACTGAAAGGCTTGGAGAATTGAGGAGCTGATGAGCGGATGAAATTAGGGT
TTAAATCGAGCTGAGAAATGGGATTTTTGGGAATGGAAGAAGAAG

Last Updated on 06.12.2018 16:11