Sequences from confirmation amplicon


Confirmation sequences for line 414H06 and hit At5g01445 are shown.
Line ID 414H06
Locus At5g01445 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:182229
Primers used for PCR 09xa - 8474, show primer details
   
Sequence Name 56-K118966-0022-414-H06C-09xa
GenBank Accession LN509254 [GenBank]
Sequencing primer gene specific primer, 09xa: 5'-TGCTCTTTGAGAAATGTCTTAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K118966-0022-414-H06C-09xa
TGTTAAGTCATACTTAAGCTAAGCATAGCAGGGTTTAAGTTACATATCTTGAAGAACAAG
GGGAGACAAAGATTTCTCAAGGGAAAATCTCATTACATAAAAAAATAAAATAAAAGGTTT
CAAGTCTTAATAAAGCATTGCAAGTTTTTTTTAGGTGATGGTGGCTTCACGAGCATCTTC
TTCTTCTTCTTCATTTTAAAAGCTTTGTGCTTGTGGAGGAATCGCATCTTTGAACTTGAT
TGAAACTGCTTAGCTACGCTGAGTCAGGTGAAAAACACTAACACATATGAGCAACAACCT
CGTCGCTAATTCAAAGCCGATCGAGAGGTAAAATGAATTGAATACCTTGTCGAAGAGTCA
CTAACATCAGGTAACATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATATCCG
   
Sequence Name 32-K118965-0022-414-H06C-8409
GenBank Accession LN500777 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118965-0022-414-H06C-8409
TCATTTTACCTCTCGATCGGCTTTGAACCTTTGACAAGGTTGTTGCTCATATGTGTTAGT
GTTTTTCACCTGACTCAGCGTAGCTAAGCAGTTTCAATCAAGTTCAAAGATGCGATTCCT
CCACAAGCACAAAGCTTTTAAAATGAAGAAGAAGAAGAAGATGCTCGTGAAGCCACCATC
ACCTAAAAAAAACTTGCAATGCTTTATTAAGACTTGAAACCTTTTATTTTATTTTTTTAT
GTAATGAGATTTTCCCTTGAGAAATCTTTGTCTCCCCTTGTTCTTCAAGATATGTAACTT
AAACCTGCTAGGCTAAGCTTAAGTATGACTTAACAATCCTTTTATTTCGGATCTTTGTCT
CACTCTATAATTGCTCAGACATTTCTGAAAGAA

Last Updated on 06.12.2018 16:11