Sequences from confirmation amplicon


Confirmation sequences for line 691E01 and hit At5g01550 are shown.
Line ID 691E01
Locus At5g01550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:215228
Primers used for PCR EU37 - 8474, show primer details
   
Sequence Name 11-K103500-0022-691-E01C-EU37
GenBank Accession LN491078 [GenBank]
Sequencing primer gene specific primer, EU37: 5'-CAAATACCTAAGCACCGTTCTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K103500-0022-691-E01C-EU37
TCTTTGATGGCACAGAGCAATCCCACAACAAGAGCAAGCCGTGCCTCAACGCCGTCGTAA
CCAAATCCAAGTCTCGGATCCACCGCATGGAGAATCTCTCCTCTCGCGTGCAATTCCATA
ACCCAATCGGCCAAGAAGAATGTCCCTGAGTCAGTCGGTCTTCTCCCGGAGACTATCTCT
AATAACAAAACGCCAAACGCAAAAACATCAGACGCAGACGAAGATTTGCCGTTGCGTGCT
AGCTCTGGTGCCATGTACCCAATTGTGCCCACGACTACGGTCGTGTTCGATTGCGATCCG
CGTTCGTAAAGCCTCGCGAGTCCAAAATCTCCCAACCTAGGATTCATATCGTCCTCAATC
AAAACGTTGCTAGGTTTTATGTCTCTGTGAATCACAACCTTTTCCCATTCTTCATGGAGA
TACAACAAACCAGACGCGATTCCTTTAGCAATCTTAAAACGTGCGTTCCACGACAAAACA
AGAAAAAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATTATAT
   
Sequence Name 27-K103501-0022-691-E01C-8409
GenBank Accession LN498349 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K103501-0022-691-E01C-8409
TTTTCTTGTTTTGTCGTGGAACGCACGTTTTAAGATTGCTAAAGGAATCGCGTCTGGTTT
GTTGTATCTCCATGAAGAATGGGAAAAGGTTGTGATTCACAGAGACATAAAACCTAGCAA
CGTTTTGATTGAGGACGATATGAATCCTAGGTTGGGAGATTTTGGACTCGCGAGGCTTTA
CGAACGCGGATCGCAATCGAACACGACCGTAGTCGTGGGCACAATTGGGTACATGGCACC
AGAGCTAGCACGCAACGGCAAATCTTCGTCTGCGTCTGATGTTTTTGCGTTTGGCGTTTT
GTTATTAGAGATAGTCTCCGGGAGAAGACCGACTGACTCAGGGACATTCTTCTTGGCCGA
TTGGGTTATGGAATTGCACGCGAGAGGAGAGATTCTCCATGCGGTGGATCCGAGACTTGG
ATTTGGTTACGACGGCTTTGAGGCACGGCTTGCTCTTGTTGTGGGATTGCTCTGTTGCCA
TCAAAGACCGACGTCTCGGCCGTCCATGAGAACGGTGCTTAGGTATTTGATGGCAACAGA
GCAATCCCACAACAAGAGCAAGCCGTGCCTCAACGCCGTCGTAACCAAATCGAAGTCTCG
GATCCACCGCATGGAGAATCTCTCCTCTCGCGTGCAATTCCATAACCCAATCGGCCAAGA
AG
Primers used for PCR 49wl - 8474, show primer details
   
Sequence Name 51-K118446-0022-691-E01C-49wl
GenBank Accession LN507640 [GenBank]
Sequencing primer gene specific primer, 49wl: 5'-GTGCAAGAAGAAATGTATGTTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118446-0022-691-E01C-49wl
CATGGGCTGGAGTTTTTCTAGCGGCGGTGATCTTCTAACTGAAGATACGCTTGACCTCTT
GGAACTTCCTCGTCCGCCTCCGAACACGGCGAAGAAGAGAGGTTACAATTCTCAAGTACT
CGCTCTGATCGTAGCTCTATCGGGAGTAACAGTGATCTTGCTTGCGTTACTCTTCTTTTT
CGTGATGTACAAGAAACGATTGCAACAAGGAGAGGTTCTTGAAGACTGGGAAATCAATCA
TCCTCATAGACTTAGATATAAAGATCTCTACGCTGCTACGGATGGATTCAAGGAGAATAG
GATTGTCGGAACTGGAGGATTTGGAACCGTTTTCCGAGGAAACTTATCGTCACCTTCGTC
TGATCAAATCGCCGTGAAGAAGATAACTCCGAATAGTATGCAAGGTGTTCGAGAATTCAT
CGCAGAGATCGAGAGTTTGGGGAGATTAAGGCATAAGAATCTGGTCAACCTTCAAGGATG
GTGCAAGCAAAAAAACGATCTCTTGTTAATTTATGATTATATCCCTAACGGAAGCTTAGA
CTCGCTGCTCTACAGTAGACCAGCAATGAGTATCTACAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 11-K118445-0022-691-E01C-8409
GenBank Accession LN491328 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118445-0022-691-E01C-8409
TTACAATTGAATATATCCTGTAGATACTCATTGCTGGTCTACTGTAGAGCAGCGAGTCTA
AGCTTCCGTTAGGGATATAATCATAAATTAACAAGAGATCGTTTTTTTGCTTGCACCATC
CTTGAAGGTTGACCAGATTCTTATGCCTTAATCTCCCCAAACTCTCGATCTCTGCGATGA
ATTCTCGAACACCTTGCATACTATTCGGAGTTATCTTCTTCACGGCGATTTGATCAGACG
AAGGTGACGATAAGTTTCCTCGGAAAACGGTTCCAAATCCTCCAGTTCCGACAATCCTAT
TCTCCTTGAATCCATCCGTAGCAGCGTAGAGATCTTTATATCTAAGTCTATGAGGATGAT
TGATTTCCCAGTCTTCAAGAACCTCTCCTTGTTGCAATCGTTTCTTGTACATCACGAAAA
AGAAGAGTAACGCAAGCAAGATCACTGTTACTCCCGATAGAGCTACGATCAGAGCGAGTA
CTTGAGAATTGTAACCTCTCTTCTTCGCCGTGTTCGGAGGCGGACGAGGAAGTTCCAAGA
GGTCAAGCGTATCTTCAGTTAGAAGATCACCGCCGCTAGAAAAACTCCAGCCCATGACGT
AGTGAGCACTCGACTGATCTCTCCCTGTAGCTGCTGTGAATCCAACATACAT

Last Updated on 06.12.2018 16:11