Sequences from confirmation amplicon


Confirmation sequences for line 362D02 and hit At5g02120 are shown.
Line ID 362D02
Locus At5g02120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:419388
Primers used for PCR GE23 - 8474, show primer details
   
Sequence Name 09-K033717-022-362-D02C-GE23
GenBank Accession LN490075 [GenBank]
Sequencing primer gene specific primer, GE23: 5'-AGGCCATGATTTTGTTTTACTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K033717-022-362-D02C-GE23
GGAAATGTGTTGCAGGATATAACACGTGGCGAAATATGAATGGATCGGAGAAAGAGAGCA
GTGTAGCCACAAGCTAAATCCTGAAGTGATTTCTGAAGATAAAGATTGTTCATATCAAGT
AGTTTTCGTTAATTCACTCTACAAAGGAAAATAATGAGCTCGTCGCCGTTATCTTCATCT
CTCTTTCATCCTCTGTCGACCTTGTCGACTCATTGCCATGGTCGGAGACAGAACCTCTGT
TTCAATCGGAAGCAACAACCTTTCGTTGTCAGAGCCGCAAAACTTCCTGAAGGGGTAACG
TTTGGAACATTTTTTCAAATATTCAAATGTCTATCTTCAATGGTAGTTAAATCGTGTTTT
TCGCAGGTGATAGTGCCAAAAGCACAACCCAAATCTCAACCTGCGTTTCTGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCAAAATGGG
   
Sequence Name 89-K033717-022-362-D02C-8409
GenBank Accession LN514914 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K033717-022-362-D02C-8409
TGAGATTTGGGTTGTGCTTTTGGCACTATCACCTGCGAAAAAACCATTTAACTACCATTG
AAGATAGACATTTGAATATTTGAAAAAATGTTCCAAACGTTACCCCTTCAGGAAGTTTTG
CGGCTCTGACAACGAAAGGTTGTTGCTTCCGATTGAAACAGAGGTTCTGTCTCCGACCAT
GGCAATGAGTCGACAAGGTCGACAGAGGATGAAAGAGAGATGAAGATAACGGCGACGAGC
TCATTATTTTCCTTTGTAGAGTGAATTAACGAAAACTACTTGATATGAACAATCTTTATC
TTCAGAAATCACTTCAGGATTTAGCTTGTGGCTACACTGCTCTCTTTCTCCGATCCATTC
ATATTTCGCCACGTGTTATATCCTGCAACACATTTTTCCTTTTTTTGCTAGAATTTTCTG
ACAGTAAAACAAAATCATGGC

Last Updated on 06.12.2018 16:11