Sequences from confirmation amplicon


Confirmation sequences for line 148H05 and hit At5g03885 are shown.
Line ID 148H05
Locus At5g03885 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1041704
Primers used for PCR 66zl - 3144, show primer details
   
Sequence Name 03-K130469-0022-148-H05C-66zl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 66zl: 5'-CAAATTCATTAAATAGGGGCTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K130469-0022-148-H05C-66zl
AGGTTTGTTTGATTTTTGTATTTTAGAGTGGTTGTTATTTTCTTGTGTGTGTCTAGCGAT
TTGTTGTAGGTTGCTGTAGGAACTACACTAGTCACGCATTGGGCGCTATGCTCACTCACA
CGCCCATCTATACAACCTATACATACAACTATACAATCTCCCCCTATTTATACATCTATA
CAATCACACCCTAATAATACATCTATACTATCACACCCTAACATACATCTAAACCATCGC
ACCCTAAACATTCATCTATTTATACATTCACACCCTAAACATACATCTATATACTCACAC
CCTATCATACATCTACACATTCACACCCTAAACATTATCATTCACATCTACATACATACA
TGTATCTTTATCCGGAAAGAAAGAGGAAAATACAAAATATTGCACAGTCTTGTCAACTTC
AAAGGCTGGCACAGCAGAAAATGGAAAATACTGGATATAGTCCCAAGGCTTCGTTTTCCC
TTCTTCAACCTTCTGCAAAATTAATTAATCAAAATCAGTATTTCGTTTTTTCCGTTGCAC
ATGCATTTACATACAAATCAAACATTTCAGTCAAAAATTAAAACCCATTCTTCCTATGTT
TTACATAAAAACTAAAATCTTCATTCTATGTTTTCCTAGACAGAACATAAATATATCTAG
CTTAATTTCAACAGCAACAAAAAGAACATATATCTAGATATAAAATTTCAGTTTATTTAA
GACACATATGTAGCTAAAAATCAGTGTATGTCATAGTTCATAGATGAAGTCAAGACAGCA
ATGTATATAAAACAGAACATTAATTTAAATTCCAAGTTCAACTCCATAAAAGATGTTAAA
AAGATGAAGAACATATCTATAAGCTAACAAGAACCGTTTATATGA
   
Sequence Name 01-K130468-0022-148-H05C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K130468-0022-148-H05C-3144
TTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATTTTTACTATTG
TTCTAACGTATAGTTTGATTTGACTTGTTGACAGTGGAAAAAATATGAACGTTGTGAAAC
GGTAACATTTTTCTTCCCGTTTGAAATTATAATATTTTATTGGAAAGAGATTCAGATAAT
GCCTCTCTATAAATACTCCTTTCTTGAATCAGAAATCTGAATCCTCTTTACTTGGTTATT
TTCTTACAAATGTCGAATACAGTCTTTTTTTTTAAAAAATTCCGTTCTTCTACTTTAGGA
GTTCTCGTAGTCAAACTTGTCTACATTGCTCATTAGCCGAGAGAAACAAACATAAGCATC
CTCTCTTACAAGGTAAAAATACTCTTGTCTTAGTTATTCTAGTTTTTTTTTTAATCATAT
AAACGGTTCTTGTTAGCTTATAAATATGTTCTTCATCTTTTTAACATCTTTTATGGAGTT
GAACTTGGAATTTAAATTAAGGTTCTGTTTTATATACATTGCTGTCTTGACTTCATCTAT
GAACTATGACATACACTGATTTTTAGCTACATATGTGTCTTAAATAAACTGAAATTTTAT
ATCTAGATATATGTTCTTTTTGTTGCGGTGGAAATTAAGCTAGATATATTTATGTTCTGT
CTAGGAAAACATAGAATGAAGATTTTAGTTTTTATGTAAAACATAGGAAGAATGGGTTTT
AATTTTTGACTGAAATGTTTGATTTGTATGTAAATGCATGTGCAACGGAAAAAACGAAAT
ACTGATTTTGATTAAATAATTTTGCAAAAGGTTAAAAAA

Last Updated on 06.12.2018 16:11