Sequences from confirmation amplicon


Confirmation sequences for line 084G06 and hit At5g05510 are shown.
Line ID 084G06
Locus At5g05510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1632293
Primers used for PCR AK17 - 3144, show primer details
   
Sequence Name 02-K105632-0022-084-G06C-AK17
GenBank Accession LN487033 [GenBank]
Sequencing primer gene specific primer, AK17: 5'-AGCTCAAACTGGCATTATAGCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K105632-0022-084-G06C-AK17
AACAGAAAGATTTGATTGATATATGTTGCATATGCATACTCACTCGAATACATTTTTCTA
GAGTTTCCAAGAATACTTACTTTTGAACCCCAAGACGGTACATTTTCTCAGCATCCTCAA
ATCTCTTCATCTTCTCATAATGGAGAGCATATGCCTGGTAGAAGAGTGACCTCTTGGTCC
CTATACTTTTTGCTTCCATGGTTCTTAAAAGAGCTCTTGGATCGTCTACAAAGTCCATCT
ACCCAAACAATTCATCATCACCCATTAAGACCCACATGCTAACGAATCAACATCTGCTAT
CAAAATCAAGTGCAAAATCAAGGGGTGAATGTACATTACCAGCTGCAACCAAACACGAAT
ATACCGCGAATCGTTCTTATATCTCTTATCAGATTCAAATGACTCTGCACATTTCTGCAA
AAACCGCGGCAGCTTCTCGTTCAGCAATATATTCAATTGTAAGGCGGAGTTTAAACCGAA
GGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCC
TTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGT
GGAGATAT
   
Sequence Name 18-K105633-0022-084-G06C-3144
GenBank Accession LN494305 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K105633-0022-084-G06C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTCCGCCTTACAATT
GAATATATTGCTGAACGAGAAGCTGCCGCGGTTTTTGCAGAAATGTGCAGAGTCATTTGA
ATCTGATAAGAGATATAAGAACGATTCGCGGTATATTCGTGTTTGGTTGCAGCTGGTAAT
GTACATTCACCCCTTGATTTTGCACTTGATTTTGATAGCAGATGTTGATTCGTTAGCATG
TGGGTCTTAATGGGTGATGATGAATTGTTTGGGTAGATGGACTTTGTAGACGATCCAAGA
GCTCTTTTAAGAACCATGGAAGCAAAAAGTATAGGGACCAAGAGGTCACTCTTCTACCAG
GCATATGCTCTCCATTATGAGAAGATGAAGAGATTTGAGGATGCTGAGAAAATGTACCGT
CTTGGGGTTCAAAAGTAAGTATTCTTGGAAACTCTAGAAAAATGTATTCGAGTGAGTATG
CATATGCAACATATATCAATCAAATCTATTCTTGGTTTTGTGGATATGAGGAATGCTATA
ATGC

Last Updated on 06.12.2018 16:11