Sequences from confirmation amplicon


Confirmation sequences for line 919B11 and hit At5g05510 are shown.
Line ID 919B11
Locus At5g05510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1631309
Primers used for PCR AK18 - 8474, show primer details
   
Sequence Name 45-K105634-0022-919-B11C-AK18
GenBank Accession LN505533 [GenBank]
Sequencing primer gene specific primer, AK18: 5'-AATTGTTTGGGTAGATGGACTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K105634-0022-919-B11C-AK18
ATATGGACCACGATGTCATATCTTCTACCAGGCATATGCTCTCCATTATGAGAAGATGAA
GAGATTTGAGGATGCTGAGAAAATGTACCGTCTTGGGGTTCAAAAGTAAGTATTCTTGGA
AACTCTAGAAAAATGTATTCGAGTGAGTATGCATATGCAACATATATCAATCAAATCTAT
TCTTGGTTTTGTGGATATGAGGAATGCTATAATGCCAGTTTGAGCTAGCATATGTGATAT
GTGGAAGACATGATTTAAATTGAGTGACTATTTCCTCTTGTTCCCAGCCTTGCAGAACCC
ATGGATGAGTTACAGAAATCATACTTGCAGTTCGTTACTCGCATGGAGAGGCACAAAAAG
AAGAAGACACAGGTACTGTTTCCATAATTTGATTGCTCCGTTTGTATAAGGGTTACAATT
TCTGCAGATGTCTGAATTTATGTTTCCTCTTGCAGCGTCAAGAACAAAAACTGTCTGGAA
AGCACCACAAGGTTGATGAGAGGCAACAACATGAGCCTGTGTTGAATTTTGTTGATAAAG
CCATTGTAGGGAAGCCTGAAGCAGAAAATGCTTGCCATCACGGTTTGGTGGATCCCACAA
TAAACATGAAGGAAGCCATGAATACAATCAACAACATGTTCAAAGAGCCTATAGAAACTG
CTCCACTTCAGAGAAGATCACGACAGAGAAGTCAGAACAAAGAAAATCAAGGATGCAACA
ATAGCTTTGAGGTTTTTCTGGACGAGAATCTCGAATGTGAAACAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 61-K105635-0022-919-B11C-8409
GenBank Accession LN510521 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K105635-0022-919-B11C-8409
CTCAAGCTATTGTTGCATCCTTGATTTTCTTTGTTCTGACTTCTCTGTCGTGATCTTCTC
TGAAGTGGAGCAGTTTCTATAGGCTCTTTGAACATGTTGTTGATTGTATTCATGGCTTCC
TTCATGTTTATTGTGGGATCCACCAAACCGTGATGGCAAGCATTTTCTGCTTCAGGCTTC
CCTACAATGGCTTTATCAACAAAATTCAACACAGGCTCATGTTGTTGCCTCTCATCAACC
TTGTGGTGCTTTCCAGACAGTTTTTGTTCTTGACGCTGCAAGAGGAAACATAAATTCAGA
CATCTGCAGAAATTGTAACCCTTATACAAACGGAGCAATCAAATTATGGAAACAGTACCT
GTGTCTTCTTCTTTTTGTGCCTCTCCATGCGAGTAACGAACTGCAAGTATGATTTCTGTA
ACTCATCCATGGGTTCTGCAAGGCTGGGAACAAGAGGAAATAGTCACTCAATTTAAATCA
TGTCTTCCACATATCACATATGCTAGCTCAAACTGGCATTATAGCATTCCTCATATCCAC
AAAACCAAGAATAGATTTGATTGATATATGTTGCATATGCATACTCACTCGAATACATTT
TTCTAGAGTTTCCAAGAATACTTACTTTTGAACCCCAAGACGGTACATTTTCTCAGCATC
CTCAAATCTCTTCATCTTCTCATAATGGAGAGCATATGCCTGGTAGAAGAGTGACCTCTT
GGTCCCTATACTTTTTGCTTCCATGGTTCTTAAAAGAGCTCTTGGATCGTCTACAAAGTC
CATCTA

Last Updated on 06.12.2018 16:11