Sequences from confirmation amplicon


Confirmation sequences for line 548B08 and hit At5g05520 are shown.
Line ID 548B08
Locus At5g05520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1632828
Primers used for PCR JL54 - 8474, show primer details
   
Sequence Name 35-K101384-0022-548-B08C-8409
GenBank Accession LN501764 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K101384-0022-548-B08C-8409
CGGACTGAGCCTTTACATTGTAAACGCTTTTTGGAGGAGCCGTCAAATTGACTTTACCGA
TTTGCTCATCTCTGACTCTCAGTTGCTTTGCTCCTTCTCATGGAGAATCCGGCGGAGAAA
CCCGACCCGAATCCATCAAAACCCAAAATCGAATCTGAAGATGAAAGAGAAGAACTAGGA
GATATTAACGGCGACGAAGAAGAAGAGGAAGAGTATGAGGAAGAAGACGATGGGAAACCT
CGAACGCGAGAGGATGCAATCGCGGATAGGATCAAAGCTGAGTCTCTGTTCCGTCGTATG
CGAGCAACTCCGGTCGCTGTGCGTGTTCACGACGTGATCGTCAAGGGAAACGAGAAGACT
AAGGATCATGTCATCGAGGCTGAGGTGGATGTTGTGAGGCAAGCCACTACGTTGCAGGAG
CTTCTCAAAGCCTCTNAGGTTGCCAATTTCAATCTACAAGCGCTTGATATCTTCGATTCA
GTCAAAATTACGCTTGATTCTGGTCCTCCTGAGCTTCCTGGTACCACCAATGTTGTTATC
GATGTTGTTGAGAGCAAAAGCCCTATCACCGGTCAAATCGGAACCTTCACTAAAGCAGAG
GTTTCACTTTCGTTTTCCTATTTCAGTGG
   
Sequence Name 35-K101394-0022-548-B08C-JL54
GenBank Accession LN501765 [GenBank]
Sequencing primer gene specific primer, JL54: 5'-TCCTGCCAAGACCTAAGTATCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K101394-0022-548-B08C-JL54
AGTGATACCTCTGCTTTATGAAGGTTCCGATTTGACCGGTGATAGGGCTTTTGCTCTCAA
CAACATCGATAACAACATTGGTGGTACCAGGAAGCTCAGGAGGACCAGAATCAAGCGTAA
TTTTGACTGAATCGAAGATATCAAGCGCTTGTAGATTGAAATTGGCAACCTTAGAGGCTT
TGAGAAGCTCCTGCAACGTAGTGGCTTGCCTCACAACATCCACCTCAGCCTCGATGACAT
GATCCTTAGTCTTCTCGTTTCCCTTGACGATCACGTCGTGAACACGCACAGCGACCGGAG
TTGCTCGCATACGACGGAACAGAGACTCAGCTTTGATCCTATCCGCGATTGCATCCTCTC
GCGTTCGAGGTTTCCCATCGTCTTCTTCCTCATACTCTTCCTCTTCTTCTTCGTCGCCGT
TAATATCTCCTAGTTCTTCTCTTTCATCTTCAGATTCGATTTTGGGTTTTGATGGATTCG
GGTCGGGTTTCTCCGCCGGATTCTCCATGAGAAGGGAGCAAAGCACTGAGAGTCAGAGAT
GAGNCAATCGGCGAAATCAATTTGACGGCTCCTCCCAAAAGCGTTT

Last Updated on 06.12.2018 16:11