Sequences from confirmation amplicon


Confirmation sequences for line 295H05 and hit At5g05550 are shown.
Line ID 295H05
Locus At5g05550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1639252
Primers used for PCR 03uc - 8474, show primer details
   
Sequence Name 39-K117317-0022-295-H05C-03uc
GenBank Accession LN503496 [GenBank]
Sequencing primer gene specific primer, 03uc: 5'-TGCTTCAGGTAAGAAGAACTCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K117317-0022-295-H05C-03uc
GGTTGTTACTCTTTCTCTTTAACCGCATAATGTATCCGTGGAAGGACCTTAGATTTCAAC
AACAATGTTATACCTATGCTGTTAGTTGTTGTAAGCATGGATGAGGCTGGTTAGTAATTG
ACTTATTGTTGATATGTGAAAGTCATGTGCCTCTCTGCTTTTGAAGAATACCAATTTTGT
CAATATACAATTCAGTTGAGCTTCTGTGCTTCCAGTGGTCTGGCAAGGAAAAGTCTAGAT
AGCATTCTCTTACTTCTTTAGCTTCTCCAGCTTCGTGGAATGTTGAACTGGATTAGGAGG
AGTAGACTTCCAGGTTTGAAATTGAGAAAAAAGATCAATGTTCATAGTAGTCGAACATTC
TTCATATGGTAATGAGTTCACTGAAGTTTGATCTGCCTTTATTGTACAGGTAAGCAATTG
TAGAATTAGATTGGTGGAGGAAATACCATGGTGAAGCAAAGAGGAGTTACTTGAATCTGA
AGCTCATTGAAATTGGAGGATCCTGTTACTTGTTTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 30-K117316-0022-295-H05C-8409
GenBank Accession LN499889 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117316-0022-295-H05C-8409
TTGAAACAAGTAACAGGATCCTCCAATTTCAATGAGCTTCAGATTCAAGTAACTCCTCTT
TGCTTCACCATGGTATTTCCTCCACCAATCTAATTCTACAATTGCTTACCTGTACAATAA
AGGCAGATCAAACTTCAGTGAACTCATTACCATATGAAGAATGTTCGACTACTATGAACA
TTGATCTTTTTTCTCAATTTCAAACCTGGAAGTCTACTCCTCCTAATCCAGTTCAACATT
CCACGAAGCTGGAGAAGCTAAAGAAGTAAGAGAATGCTATCTAGACTTTTCCTTGCCAGA
CCACTGGAAGCACAGAAGCTCAACTGAATTGTATATTGACAAAATTGGTATTCTTCAAAA
GCAGAGAGGCACATGACTTTCACATATCAACAATAAGTCAATTACTAACCAGCCTCATCC
ATGCTTACAACAACTAACAGCATAGGTATAACATTGTTGTTGAAATCTAAGGTCCTTCCA
CGGATACATTATGCGGTTTAAAGAGAAAGAGTAACAACCATGTAAAGTCTAAGCCACCAC
TAGTAACTTGAAAACCTAATGTGAGTTCTT

Last Updated on 06.12.2018 16:11