Sequences from confirmation amplicon


Confirmation sequences for line 552A03 and hit At5g08460 are shown.
Line ID 552A03
Locus At5g08460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2734842
Primers used for PCR 14xb - 8474, show primer details
   
Sequence Name 68-K118874-0022-552-A03C-14xb
GenBank Accession LN512107 [GenBank]
Sequencing primer gene specific primer, 14xb: 5'-TCCTCTATTTCTCCCTACTCCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K118874-0022-552-A03C-14xb
ACATAAAAGAAACAAAATCAAACCATTTCAAGTTAAAAATGCTCTCAACTAAGCTAAAAC
GAAACATTTGATTAAAAAAAATGTAAAACAAGGGATAAGTTAGATACTTAACCGTAATTA
AATGGGTTGGTGAGAATATCCACGGCGGCTCCATAGGTATTACCGTAAACAAAGATGGCT
TCACTAGCGGTTTTGTTATCGGAATTGAGACGATCCACCAGCGATACAAGACGGTTGTTG
AAAAGTTCAGCCATCTCGTTTACAGCCTCGACACATTCACCGGGAAATCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 28-K118873-0022-552-A03C-8409
GenBank Accession LN499062 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118873-0022-552-A03C-8409
ACATTGATTTCCCGGTGAATGTGTCGAGGCTGTAAACGAGATCGCTGAACTTTTCAACAA
CCGTCTTGTATCGCTGGTGGATCGTCTCAATTCCGATAACAAAACCGCTAGTGAAGCCAT
CTTTGTTTACGGTAATACCTATGGAGCCGCCGTGGATATTCTCACCAACCCATTTAATTA
CGGTTAAGTATCTAACTTATCCCTTGTTTTACATTTTTTTTAATCAAATGTTTCGTTTTA
GCTTAGTTGAGAGCATTTTTAACTTGAAATGGTTTGATTTTGTTTTCTTTTATTGTTTGT
AGGATTCGAAGTGACGGATAGAGGGTGTTGTGGAGTAGGGAGAAAAATAGAGGA

Last Updated on 06.12.2018 16:11