Sequences from confirmation amplicon


Confirmation sequences for line 659D07 and hit At5g08740 are shown.
Line ID 659D07
Locus At5g08740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2851163
Primers used for PCR HM60 - 8474, show primer details
   
Sequence Name 56-K101220-0022-659-D07C-8409
GenBank Accession LN509043 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K101220-0022-659-D07C-8409
CGGACTGAGCCTTTACATTGAAATTTAGATCCAAGTGTGAAACTTTTGAAAGCTTGATTA
GTAAAGATGATATTCTAGATGAAGATTGAAACTTTTGGTGTTATTGTCTCAATGTGTTTC
AGATGGGGCAACGAGACTGACTAGTAAAGCTTCATTAGCCTCAAGAACTTCCGGATTCAA
TCTCTCTTCTCGTTGGAACTCAACGAGAAACTCCCCTATGTTGTATCTTTCGAGAGCAGT
GACAAACAACAGTGGCACCACAGAGATTTCTGATAATGAAACAGCTCCAAGGACTTACTC
ATGGCCTGATAACAAGGTTCGTAATTCTCTAGAACGATGTATGAGATAGAAAAAGTTCAA
TTGCTGATCCCGTAACTTAATCATG
   
Sequence Name 56-K101227-0022-659-D07C-HM60
GenBank Accession LN509044 [GenBank]
Sequencing primer gene specific primer, HM60: 5'-ACATGATTAAGTTACGGGATCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K101227-0022-659-D07C-HM60
CGTTCTGAGAATACGAACCTTGTTATCAGGCCATGAGTAAGTCCTTGGAGCTGTTTCATT
ATCAGAAATCTCTGTGGTGCCACTGTTGTTTGTCACTGCTCTCGAAAGATACAACATAGG
GGAGTTTCTCGTTGAGTTCCAACGAGAAGAGAGATTGAATCCGGAAGTTCTTGAGGCTAA
TGAAGCTTTACTAGTCAGTCTCGTTGCCCCATCTGAAACACATTGAGACAATAACACCAA
AAGTTTCAATCTTCATCTAGAATATCATCTTTACTAATCAAGCTTTCAAAAGTTTCACAC
TTGGATCTAAATTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATT

Last Updated on 06.12.2018 16:11