Sequences from confirmation amplicon


Confirmation sequences for line 507G05 and hit At5g09450 are shown.
Line ID 507G05
Locus At5g09450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2943210
Primers used for PCR CC76 - 8474, show primer details
   
Sequence Name 27-K106287-0022-507-G05C-CC76
GenBank Accession LN498398 [GenBank]
Sequencing primer gene specific primer, CC76: 5'-GTTCATGTGCTGCAACCTTTAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K106287-0022-507-G05C-CC76
TGAGAGATGAGGCTGATGCTAGTTCCATGAGGGTTGGGTTCGGTATATCGATCTTACTAG
CATCTACATAAACAGTAGTAGAGTAACTAATGCAGAGTCTACTTTGCCTGTCGAAGCTGA
GAAATCTATTTCGCAAAGAGAATGGATCACATATGACTTCCTTATGATTCTGCACACTGG
TCTAGGGAACAAGGTTATGATAGATCAGATTTGGAAGTCTTTGAGAAACACCAACCAGAT
ATTGAGTAGTAGAAGCTACATTTGTGTTCTTTCATCTTATCTAATGCTTGGTCATTTGAG
AGAAGCCGAGGAGATTATCCATCAGTGGAAAGAGTCTAAAACGACAGAGTTTGATGCTTC
TGCTTGCTTGAGGATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 27-K106286-0022-507-G05C-8409
GenBank Accession LN498397 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K106286-0022-507-G05C-8409
GCAGCAGAACATCAAACTCTGTCGTTTAGACTCTTTCCCTGATGGATAATCTCCTCGGCT
TCTCTCAAATGACCAAGCATTAGATAAGATGAAAGAACACAAATGTAGCTTCTACTACTC
AATATCTGGTTGGTGTTTCTCAAAGACTTCCAAATCTGATCTATCATAACCTTGTTCCCT
AGACCAGTGTGCAGAATCATAAGGAAGTCATATGTGATCCATTCTCTTTGCGAAATAGAT
TTCTCAGCTTCGACAGGCAAAGTAGACTCTGCATTAGTTACTCTACTACTGTTTATGTAG
ATGCTAGTAAGATCGATATACCGAACCCAACCCTCATTGGAACTAGCATCATGCCTCATC
TCTTCAAGAATCTTTCTAAGCTCATCTATATTAAAGGTTGCAGCACATGAA

Last Updated on 06.12.2018 16:11