Sequences from confirmation amplicon


Confirmation sequences for line 765G02 and hit At5g10510 are shown.
Line ID 765G02
Locus At5g10510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3319143
Primers used for PCR NA61 - 8474, show primer details
   
Sequence Name 12-K101562-0022-765-G02C-OriL
GenBank Accession LN491512 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K101562-0022-765-G02C-OriL
TCGGACTGAGCCATTTACAATTGAATATATCCTGACAGGAGGTAAGTCCAAAAGCAAAAA
CTCTTTAAAATCTTATCAATGGTTTTATATTATGTTTCTTTGATGGTAACCAGAGTTTAA
AAACTGATATAACACAGGAAGAGTAGCGGTTTCTCTAGAGGAGCTTCGATATACCGAGGT
GTTACAAGGCATCATCAACAAGGACGTTGGCAAGCAAGGATTGGCCGAGTTGCTGGGAAC
AAAGATCTTTACCTTGGAACCTTTGGTATGTAAAATCTTCAAACTTGGTCTAGCTAATTT
AACAATCATTGTCTGTGGTTAATTAGTTAGTCCTGGTGTTGTTGTTGTA
   
Sequence Name 12-K101571-0022-765-G02C-NA61
GenBank Accession LN491513 [GenBank]
Sequencing primer gene specific primer, NA61: 5'-GCTACAACAACAACACCAGGACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K101571-0022-765-G02C-NA61
TTAATTGCTAGACCAAGTTTGAAGATTTTACATACCAAAGGTTCCAAGGTAAAGATCTTT
GTTCCCAGCAACTCGGCCAATCCTTGCTTGCCAACGTCCTTGTTGATGATGCCTTGTAAC
ACCTCGGTATATCGAAGCTCCTCTAGAGAAACCGCTACTCTTCCTGTGTTATATCAGTTT
TTAAACTCTGGTTACCATCAAAGAAACATAATATAAAACCATTGATAAGATTTTAAAGAG
TTTTTGCTTTTGGACTTACCTCCTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATNTCCGC
Primers used for PCR NA59 - 8474, show primer details
   
Sequence Name 28-K101669-0022-765-G02C-8409
GenBank Accession LN498779 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K101669-0022-765-G02C-8409
CGGACTGAGCATTTACATTGAATATATCCTGACAGGAGGTAAGTCCAAAAGCAAAAACTC
TTTAAAATCTTATCAATGGTTTTATATTATGTTTCTTTGATGGTAACCAGAGTTTAAAAA
CTGATATAACACAGGAAGAGTAGCGGTTTCTCTAGAGGAGCTTCGATATACCGAGGTGTT
ACAAGGCATCATCAACAAGGACGTTGGCAAGCAAGGATTGGCCGAGTTGCTGGGAACAAA
GATCTTTACCTTGGAACCTTTGGTATGTAAAATCTTCAAACTTGGTCTAGCTAATTTAAC
AATCATTGTCTGTGGTTAATTAGTTAGTCCTGGTGTTGTTGTTGTAG
   
Sequence Name 28-K101675-0022-765-G02C-NA59
GenBank Accession LN498780 [GenBank]
Sequencing primer gene specific primer, NA59: 5'-GCTACAACAACAACACCAGGACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K101675-0022-765-G02C-NA59
TGATTGTTAATAGCTAGACAAGTTTGAAGATTTTACATACCAAAGGTTCCAAGGTAAAGA
TCTTTGTTCCCAGCAACTCGGCCAATCCTTGCTTGCCAACGTCCTTGTTGATGATGCCTT
GTAACACCTCGGTATATCGAAGCTCCTCTAGAGAAACCGCTACTCTTCCTGTGTTATATC
AGTTTTTAAACTCTGGTTACCATCAAAGAAACATAATATAAAACCATTGATAAGATTTTA
AAGAGTTTTTGCTTTTGGACTTACCTCCTGTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATA

Last Updated on 06.12.2018 16:11