Sequences from confirmation amplicon


Confirmation sequences for line 671G06 and hit At5g11180 are shown.
Line ID 671G06
Locus At5g11180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3557819
Primers used for PCR 31nb - 8474, show primer details
   
Sequence Name 46-K114597-0022-671-G06C-31nb
GenBank Accession LN505940 [GenBank]
Sequencing primer gene specific primer, 31nb: 5'-GGAACAGTGGAGTTAACATTGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K114597-0022-671-G06C-31nb
AACAATGGGTTACAAGAATCGAGGTTAAAGACTTATGATACTCCTCAGGAAATGCATGAG
CTTTTTCTTAAAAAGAGCAGCAATGGTGGTATTGATGCTGCATTCGACGAAGTCGCTTAT
GTCAAGCTTTTCATGGCTAAATATTGCTCAAAATATACCATTATCGAGCCTACCTTTAAG
GCTGATGGCTTTGGCTTTGTAAGTAAACCTGGTTACTTTTATCTAGGCTTTGAATAGTGG
CAGAGGATGGGGCGGGACTAGTACAAACCGCTTTTAGCTACCACTAAAAAGTATTGGTCT
ATAGCATTACGAATAAAATATAGACTCTATCCATGCTACGAAAATATGTTCAAACCATCG
AGTTTGTTCTTAATATGATTTTGAAATGTGTTTTTGGATCTCCATTTTTTTTGTAGGCCT
TTCCATTGGGAAATCTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCC
   
Sequence Name 06-K114596-0022-671-G06C-8409
GenBank Accession LN488915 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K114596-0022-671-G06C-8409
ATTTCCCAATGGAAAGGCCTACAAAAAAAATGGAGATCCAAAAACACATTTCAAAATCAT
ATTAAGAACAAACTCGATGGTTTGAACATATTTTCGTAGCATGGATAGAGTCTATATTTT
ATTCGTAATGCTATAGACCAATACTTTTTAGTGGTAGCTAAAAGCGGTTTGTACTAGTCC
CGCCCCATCCTCTGCCACTATTCAAAGCCTAGATAAAAGTAACCAGGTTTACTTACAAAG
CCAAAGCCATCAGCCTTAAAGGTAGGCTCGATAATGGTATATTTTGAGCAATATTTAGCC
ATGAAAAGCTTGACATAAGCGACTTCGTCGAATGCAGCATCAATACCACCATTGCTGCTC
TTTTTAAGAAAAAGCTCATGCATTTCCTGAGGAGTATCATAAGTCTTTAACCTCGATTCT
TTGTAACCCATTTGTTTCAGCCTTTCGAATGTAAAGGAACCAGTTTGATATCCAATGTT

Last Updated on 06.12.2018 16:11