Sequences from confirmation amplicon


Confirmation sequences for line 275E01 and hit At5g11660 are shown.
Line ID 275E01
Locus At5g11660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3749330
Primers used for PCR 45pm - 8474, show primer details
   
Sequence Name 52-K115064-0022-275-E01C-45pm
GenBank Accession LN507905 [GenBank]
Sequencing primer gene specific primer, 45pm: 5'-TATTGGGAGAAAAAGCATTTCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K115064-0022-275-E01C-45pm
CAAATACTTGAATTAATTACTTGAGTTACAAATGTGTATATTTGGAAAATATGTATGATA
TATTTTTTTTGGAAATTTGAAGTATGCTGGAGTTAAATCACAAGGTGATGGTCCTTATCA
TGGGTTGGCAGCTTATATGAGTGTGCATGATTTGAATATTAGTAGGGACCAAACTTCATA
TGCTAGCATTTTTGTTGGTAGTGGAGACAACAAGAAAATTAATTTTATTCAAACCGGTTG
GATGGTAAATTTTATGCGGCATGTAAATTACCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGTTATA
   
Sequence Name 20-K115063-0022-275-E01C-8409
GenBank Accession LN495336 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115063-0022-275-E01C-8409
CATTGGTAATTTACATGCCGCATAAAATTTACCATCCAACCGGNTTTGAATAAAATTAAT
TTTCTTGTTGTCTCCACTACCAACAAAAATGCTAGCATATGAAGTTTGGTCCCTACTAAT
ATTCAAATCATGCACACTCATATAAGCTGCCAACCCATGATAAGGACCATCACCTTGTGA
TTTAACTCCAGCATACTTCAAATTTCCAAAAAAAATATATCATACATATTTTCCAAATAT
ACACATTTGTAACTCAAGTAATTAATTCAAGTATTTTGTTTACATGTGTCCCATGACTTC
CGACGGTATGCGGGTTGAAATGCTTTTCCTCCCAAT

Last Updated on 06.12.2018 16:11