Sequences from confirmation amplicon


Confirmation sequences for line 453A08 and hit At5g13800 are shown.
Line ID 453A08
Locus At5g13800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4452733
Primers used for PCR BU19 - 8474, show primer details
   
Sequence Name 08-K104372-0022-453-A08C-BU19
GenBank Accession LN489714 [GenBank]
Sequencing primer gene specific primer, BU19: 5'-GGGTTTCTTCCCTAATCCAGTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K104372-0022-453-A08C-BU19
CCCGGACATTCCCTCTGCCGGAAGAGTGAAAAAAATCACAGAATTGGTGTAAGGGAGACT
TTATTAACACATCTCCTCACCTGAAAAGTATCATTTTCTATTTCTATCTCTATCAGTCTA
ATATCTTTACTTCCCAATGAAACTGCAGGTGGCAAAAGATAAGTGATCCTGAAAGCATAG
CTGAGATACTTAAACAGGTCTACACAGACCATTCTATCAATGTGGATAAAGTATTCTCAC
GTATTGTGGAGGTCACACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACCGGTTATT
   
Sequence Name 24-K104373-0022-453-A08C-8409
GenBank Accession LN496975 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K104373-0022-453-A08C-8409
ATTGAATAATCCTGTGTGACCTCCACAATACGTGAGAATACTTTATCCACATTGATAGAA
TGGTCTGTGTAGACCTGTTTAAGTATCTCAGCTATGCTTTCAGGATCACTTATCTTTTGC
CACCTGCAGTTTCATTGGGAAGTAAAGATATTAGACTGATAGAGATAGAAATAGAAAATG
ATACTTTTCAGGTGAGGAGATGTGTTAATAAAGTCTCCCTTACACCAATTCTGTGATTTT
TTTCACTCTTTCCGGCAGAGGGAATGCTCCGGGCCATGGAAAGAGACGTGCTAGCTTTGG
GGATCTTACTGGATTAGGGAAGAAACCCAAGGCTAGCACGTCTCTTTCCATGGCCCGGAG
CATTCNCTCTGCCG

Last Updated on 06.12.2018 16:11