Sequences from confirmation amplicon


Confirmation sequences for line 052H01 and hit At5g14650 are shown.
Line ID 052H01
Locus At5g14650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4725084
Primers used for PCR CW45 - 8474, show primer details
   
Sequence Name 72-K104127-0022-052-H01C-CW45
GenBank Accession LN512717 [GenBank]
Sequencing primer gene specific primer, CW45: 5'-GTGTATACTGGAAAGGAAACCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K104127-0022-052-H01C-CW45
GGGAGTTCCGCGACATCAATGTGCTTGACCATGGCGCACAAGGTGACGGGACAAGTGATG
ACACAAAGGTAAGTGAGCATTTCTATTGTTTAGCTGCGTTTCTATCCGTCTCATCGATAT
ATAATGGAACCGTTTGTATCGCAGGCTTTTGAAGATGCTTGGCAGGTAGCTTGTAAGGTG
GCAGCGTCAACATTATTAGTTCCATCTGGTTCTACGTTCCTTGTTGGACCTGTTTCCTTC
CTGGGAAAAGAATGTAAAGAGAAAATCGTCTTTCAGGTAATTTACTCAAGAACAAAAAAA
TGTTAATCAAGCATTTCTAGTTATATAATCGTGACTGATGTATTTGGATACTGTGTAGTT
AGAAGGAAAGATCATAGCTCCTACGAGTGCTAGTGCATGGGGATCAGGTCTCTTGCAATG
GATAGAATTCAAAGCACTTCGGCTGTAAATAATAAGAGTTAATGGCTTATTAACTCTTCG
GCTGTTTATCAATTTTGTTTATAATTGATACATGTATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 85-K104128-0022-052-H01C-8409
GenBank Accession LN514541 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K104128-0022-052-H01C-8409
AAGCCATTTACAATTGAATATACATGTATCAATTATAAACAAAATTGATAAACAGCCGAA
GAGTTAATAAGCCATTAACTCTTATTATTTACAGCCGAAGTGCTTTGAATTCTATCCATT
GCAAGAGACCTGATCCCCATGCACTAGCACTCGTTGGAGCTATGATCTTTCCTTCTAACT
ACACAGTATCCAAATACATCAGTCACGATTATATAACTAGAAATGCTTGATTAACATTTT
TTTGTTCTTGAGTAAATTACCTGAAAGACGATTTTCTCTTTACATTCTTTTCCCAGGAAG
GAAACAGGTCCAACAAGGAACGTAGAACCAGATGGAACTAATAATGTTGACGCTGCCACC
TTACAAGCTACCTGCCAAGCATCTTCAAAAGCCTGCGATACAAACGGTTCCATTATATAT
CGATGAGACGGATAGAAACGCAGCTAAACAATAGAAATGCTCACTTACCTTTGTGTCATC
ACTTGTCCCGTCACCTTTTGCGCCATGGTCAAGCACATTGATTGTTCCGGAACTTCCCCC
TTCGGCGATAGACCTTCGGTTTCCTTCCCGGTATACAC

Last Updated on 06.12.2018 16:11