Sequences from confirmation amplicon


Confirmation sequences for line 752D10 and hit At5g17440 are shown.
Line ID 752D10
Locus At5g17440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5752912
Primers used for PCR 60sm - 8474, show primer details
   
Sequence Name 52-K116491-0022-752-D10C-60sm
GenBank Accession LN507927 [GenBank]
Sequencing primer gene specific primer, 60sm: 5'-TGGTTGGTAGTCCTCATTCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K116491-0022-752-D10C-60sm
ATTTTTGAGCGTCTATGACAGGTTAACTTTAATTACTTAAATTTGTTTATGTTTTATGGT
TCCGTGGCGTTTCATAAGAAAGCTAGAAATAGATTATTGGGTTGCAAGTGGTTCATTATT
TGACCCCGGCTATTGTACCTCCTTTTCACCAGCAGGCTATTTGGGCCCAGTTTTACGAGG
ATTACTTTTGCAGCTAATAAATGTGCGTTCTTTCTTGATTTATTGTGCCTGTTAGAGATA
AATAATGGAACTTTAAACTATATCTTTGTCACCAATCTGTTATTTGCATTTGGCAATGTC
AAAAATCTGTTGTACTGCCTTTAGTGATCGTCGGTTAGCTGATCATTTTGGAGGGAAGCT
TCACTTGGGTTACATGCTGATCCGCGACAAATTGGCAGAGCTTCAGGTAATATACAGTAG
AATCTTTCTAGTGATTTTCCTGTACAGTACAATTGAATATATCCCTAAGATCAGATTGTC
GTTTCCCGCCTGATGTGTATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATAACCGCACGGTTATT
   
Sequence Name 20-K116490-0022-752-D10C-8409
GenBank Accession LN495369 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116490-0022-752-D10C-8409
ATACACATCAGGCGGGAAACGACAATCTGATCTTAGGGATATATTCAATTGTACTGTACA
GGAAAATCACTAGAAAGATTCTACTGTATATTACCTGAAGCTCTGCCAATTTGTCGCGGA
TCAGCATGTAACCCAAGTGAAGCTTCCCTCCAAAATGATCAGCTAACCGACGATCACTAA
AGGCAGTACAACAGATTTTTGACATTGCCAAATGCAAATAACAGATTGGTGACAAAGATA
TAGTTTAAAGTTCCATTATTTATCTCTAACAGGCACAATAAATCAAGAAAGAACGCACAT
TTATTAGCTGCAAAAGTAATCCTCGTAAAACTGGGCCCAAATAGCCTGCTGGTGAAAAGG
AGGTACAATAGCCGGGGTCAAATAATGAACCACTTGCAACCCAATAATCTATTTCTAGCT
TTCTTATGAAACGCCACGGAACCATAAAACATAAACAAATTTAAGTAATTAAAGTTAACC
TGTCATAGACGCTCAAAAATGCTCCGCATATGTCACATAGACGCAGCTTCTGGTCTGTCT
GAGACAATAAGAGAAGAGAGAATGAGGACACCCAACC

Last Updated on 06.12.2018 16:11