Sequences from confirmation amplicon


Confirmation sequences for line 156B07 and hit At5g17880 are shown.
Line ID 156B07
Locus At5g17880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5910270
Primers used for PCR 03fe - 8474, show primer details
   
Sequence Name 59-K111507-0022-156-B07C-03fe
GenBank Accession LN510035 [GenBank]
Sequencing primer gene specific primer, 03fe: 5'-ACCATTAACCTCAATTCCACTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K111507-0022-156-B07C-03fe
AGATCCTCATCGCATCGTTTAGCGCACTAGCAAGCAAATGACACTTCCTTTCAGCATATA
CTACAATTTCCTCCTTTGCAGCTTGTTCCAATTCATTGCAGTTAGTGAAAATGAACGAGG
AATTGACATGCTTCATCGGTATAGAACATACCAGCGGTTTTGCAACTGTCTTCAGTGAGC
TACAACCATGAACGTTTAAATACTGAAGATTTGGTGGAAGCTGTGGAACATGTGTAAGAT
TCTTACAATACTTCAAATGAAGCCATTGAAGTTGAGAAAATTTATTGAGGAGATCTGGAA
GGCGGCTGATCTTTTCATTCTTGTTTAAGCATAAACGACGCACGGAGAGTATCTTCGGCA
TATCTTTTATAGCTGTCTCATCCAGAAGCAAGATCTCTAAGCGGCTCATGTTTCCCCAAG
TTTCTGGAAACTCATTGAGCTTTGAACAACCAATTTACATGTAAAAGACTTGTTATGTAG
TTTCTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCA
   
Sequence Name 47-K111506-0022-156-B07C-8409
GenBank Accession LN506231 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K111506-0022-156-B07C-8409
TTTCATTGAATTATCCTGCAGAAACTCATAACAAGTCTTTTACATGTACCCTTTAATGTT
CAAAGCTCAATGAGTTTCCAGAAACTTGGGGAAACTTGAGCCGCTTAGAGATCTTGCTTC
TGGATGAGACAGCTATAAAAGATATGCCGAAGATACTCTCCGTGCGTCGTTTATGCTTAA
ACAAGAATGAAAAGATCAGCCGCCTTCCAGATCTCCTCAATAAATTTTCTCAACTTCAAT
GGCTTCATTTGAAGTATTGTAAGAATCTTACACATGTTCCACAGCTTCCACCAAATCTTC
AGTATTTAAACGTTCATGGTTGTAGCTCACTGAAGACAGTTGCAAAACCGCTGGTATGTT
CTATACCGATGAAGCATGTCAATTCCTCGTTCATTTTCACTAACTGCAATGAATTGGAAC
AAGCTGCAAAGGAGGAAATTGTAGTATATGCTGAAAGGAAGTGTCATTTGCTTGCTAGTG
CGCTTAAACGATGCGATGAGGTATCTCTATTCCTCTCCCTTGAGAGTTTTCTTTTGCGTA
GTGGAATTGAGGTTAATGG

Last Updated on 06.12.2018 16:11