Sequences from confirmation amplicon


Confirmation sequences for line 550A02 and hit At5g18010 are shown.
Line ID 550A02
Locus At5g18010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5963036
Primers used for PCR JL50 - 8474, show primer details
   
Sequence Name 36-K101384-0022-550-A02C-8409
GenBank Accession LN502157 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K101384-0022-550-A02C-8409
CTTTACATTGAATAATCCTGATCATATATCCAACATTTTTCCTTTAAGTTAGAAATAGAC
TTATTCATTGTAAATACATGACTCTTTTTTCTCTTTCTTCTTGAAAGAGTTGTTCTAAAA
GTGTAGAAAGTGAAATACAAAATTAATGTTATATACAAACTTCATGTCAATACATTTTTG
ATCGCTCATTGAAAAAGAAAGACGACACCAAATTCATCAATCAAAAAACTACTCTAATTA
TAGTTTCATGTTGATTAAAAACAATTAAGTGGAACATCTAACATCCGGATCCGTGGCGCA
ATGGTAGCGCGTCTGACTCCAGATCAGAAGGTTGCGTGTTCGATTCACGTCGGGTTCAAT
CCCCCGAACTTTATTCCGGATATCTTTTTGTTTTACCTGCTTATTTCCAATTTTTGCCTT
TTGATGCACTACCCCCGGTTCGGGATTCTTGTTACTCTAACCGAGAATTTCAGATCCGGT
TTTAGTATTTGTTTGCTACTCTGAATTAGACGGAAACAAATAACAAATGACCACTTTTAA
CTTACACAATTTATCAGTGGCTGGCTCTTACGTATAGTGGGGGGGTTACATTTAGTTGAT
GTCTCCCGTGACATTTGATTATAGTTTTCAAGAAATTGTCCAGCAAAAAGGAAAAGAAAA
   
Sequence Name 36-K101394-0022-550-A02C-JL50
GenBank Accession LN502158 [GenBank]
Sequencing primer gene specific primer, JL50: 5'-TTCTTTTCCTTTTTGCTTGACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K101394-0022-550-A02C-JL50
TGGAGACTCAACTAAATGTAACCCCCACACTATACGTAAGAGCCAGCCACTGATAAATTG
TGTAAGTTAAAAGTGGTCATTTGTTATTTGTTTCCGTCTAATTCAGAGTAGCAAACAAAT
ACTAAAACCGGATCTGAAATTCTCGGTTAGAGTAACAAGAATCCCGAACCGGGGGTAGTG
CATCAAAAGGCAAAAATTGGAAATAAGCAGGTAAAACAAAAAGATATCCGGAATAAAGTT
CGGGGGATTGAACCCGACGTGAATCGAACACGCAACCTTCTGATCTGGAGTCAGACGCGC
TACCATTGCGCCACGGATCCGGATGTTAGATGTTCCACTTAATTGTTTTTAATCAACATG
AAACTATAATTAGAGTAGTTTTTTGATTGATGAATTTGGTGTCGTCTTTCTTTNNTCATG
AGCGATCAAAAATGTATTGACATGAAGTTTGTATATNACATTAATTTTTGTATTCACTTT
CTACACTTTTAGAAACACTC
Primers used for PCR JL51 - 8474, show primer details
   
Sequence Name 37-K101384-0022-550-A02C-OriL
GenBank Accession LN502553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K101384-0022-550-A02C-OriL
ATTTACATTGAATATATCCTGAGCAATACATTGTAAATACAACGAATGTTGGAGCCGAGA
AGTCACATTGATGAAAGTATCTTCGGGACAAGGGATCGTTAAGCCACCCATCGGATGAGC
AAACCCAAACTCTTCTTCGGATTTACTGAGCAGAGCTTGAAATGACGGTTGGCTCAAGTA
TGAGAGCGGCACCAAATATCTCTTCTTCTGGCTCTCTCCTACGTACACCGCAAGAAACCC
TTTTGGTGCCGCAGATCCTGCTGCGGTGGAGCGGCTTAGAATCTTCTTTGCTCCCAATAG
ACTTCTCATTTTTATTTTATTTTAAATAAAAGAAGTTTGAATTATTATGAAGCTCTTGAA
GATGTATGAAAAGATTCAAGTTATTGGAATGCTTGTTGTTGGTTGATGATAGAAATAATG
GGGTTATGGGTGTATATATAGAAATATCAATGAACGATTTGAAACTTCTCTGAAAAAGTC
ATTGAAACTGTGGTCTTGAAGCGTGGGACAGACTAGAGCAAGAGTTCACATGGTTCTGTC
TTTACCATCTGGAAAAGATGCATTAATGAAATTTATCTACTTAAATAATCTCCATGTGAA
ATTTCACAAGGGCTTGCTTTCTCAATGTTTCTGAGATAAGACGTGCCCTATATTTGCCAG
AACAAAGACCGCAAGTTCTGTGATGA
   
Sequence Name 37-K101394-0022-550-A02C-JL51
GenBank Accession LN502554 [GenBank]
Sequencing primer gene specific primer, JL51: 5'-AAGAACCAACCACCTAATCATCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K101394-0022-550-A02C-JL51
AGGGCACGTCTTATCTCGAAACATTGAGAAAGCAAGCCCTTGTGAAATTTCACATGGAGA
TTATTTAAGTAGATAAATTTCATTAATGCATCTTTTCCAGATGGTAAAGACAGAACCATG
TGAACTCTTGCTCTAGTCTGTCCCACGCTTCAAGACCACAGTTTCAATGACTTTTTCAGA
GAAGTTTCAAATCGTTCATTGATATTTCTATATATACACCCATAACCCCATTATTTCTAT
CATCAACCAACAACAAGCATTCCAATAACTTGAATCTTTTCATACATCTTCAAGAGCTTC
ATAATAATTCAAACTTCTTTTATTTAAAATAAAATAAAAATGAGAAGTCTATTGGGAGCA
AAGAAGATTCTAAGCCGCTCCACCGCAGCAGGATCTGCGGCACCAAAAGGGTTTCTTGCG
GTGTACGTAGGAGAGAGCCAGAAGAAGAGATATTTGGTGCCGCTCTCATACTTGAGCCAA
CCGTCATTTCAAGCTCTGCTCAGTAAATCCGAAGAAGAGTTTGGGTTTGCTCATCCGATG
GGTGGCTTAACGATCCCTTGTCCCGAAGATACTTTCATCAATGTGACTTCTCGGCTCCCA
CATTCATTGTATTTACNATGTATTGCTC

Last Updated on 06.12.2018 16:11