Sequences from confirmation amplicon


Confirmation sequences for line 243F08 and hit At5g18525 are shown.
Line ID 243F08
Locus At5g18525 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6153657
Primers used for PCR GD34 - 8474, show primer details
   
Sequence Name 31-K101282-0022-243-F08C-GD34
GenBank Accession LN500090 [GenBank]
Sequencing primer gene specific primer, GD34: 5'-ACGTATTTAGCTAAGCAGCCATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101282-0022-243-F08C-GD34
TGACTGAGAAAAGTGGATTCACAAGAAGAACAAGAAGCAGAAAATTCCTCGCTAGAATCA
GAAACCTACACAAACGCCATTTAAAGCAGCTTAAATTCCAATACTTCCACCATTGAAATC
TACAATTCAAATAATGACAATTGAAAAATCCACCTTAACGACGGCGGAGGATCCAAAAGG
TAGCGGCGAATCGGAGACACCGTAAGAGAAGACGATCTGATCGGAGAAGTCAGAGTTGAT
ACGTTGATCGAGACAATCGAAGCAAAGGTCACTATCTTCACCTCTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 26-K101282-0022-243-F08C-8409
GenBank Accession LN497851 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K101282-0022-243-F08C-8409
TCGGACTGAGCCTTTACATTGAGAGGTGAAGATAGTGACCTTTGCTTCGATTGTCTCGAT
CAACGTATCAACTCTGACTTCTCCGATCAGATCGTCTTCTCTTACGGTGTCTCCGATTCG
CCGCTACCTTTTGGATCCTCCGCCGTCGTTAAGGTGGATTTTTCAATTGTCATTATTTGA
ATTGTAGATTTCAATGGTGGAAGTATTGGAATTTAAGCTGCTTTAAATGGCGTTTGTGTA
GGTTTCTGATTCTAGCGAGGAATTTTCTGCTTCTTGTTCTTCTTGTGAATCCACTTCTTC
TCAGTTCATTTTGGAGTATCTCCGGAAGGACGAACATGGCTGCTTACTNNAATACG
Primers used for PCR GE72 - 8474, show primer details
   
Sequence Name 32-K101282-0022-243-F08C-GE72
GenBank Accession LN500513 [GenBank]
Sequencing primer gene specific primer, GE72: 5'-TCTCCAACATGAAAAACTACCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K101282-0022-243-F08C-GE72
TCACAGTAAATTCTCTGTGTCATCATCTTCAGTAGCTTGAGAGCCACTGGTTGAACAATC
CAAACACTCATCAGATTCAACAGCATCGTTTGAATTACCCTCTCGATCCTTAACAACAAA
TTTGTCGCTGCAGAAAAAGAATAATTTGCTCAACCACAATGATTGATAGAATCAAATCGT
TAGCCTAAGGAGAACATAAACAAGAACGTACACGTATTTAGCTAAGCAGCCATGTTCGTC
CTTCCGGAGATACTCCAAAATGAACTGAGAAGAAGTGGATTCACAAGAAGAACAAGAAGC
AGAAAATTCCTCGCTAGAATCAGAAACCTACACAAACGCCATTTAAAGCAGCTTAAATTC
CAATACTTCCACCATTGAAATCTACAATTCAAATAATGACAATTGAAAAATCCACCTTAA
CGACGGCGGAGGATCCAAAAGGTAGCGGCGAATCGGAGACACCGTANGAGAAGACGATCT
GATCGGAGAAGTCAGAGTTGATACGTTGATCGAGACAATCGAAGCAAAGGTCACTATCTT
CACCTCTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCC
   
Sequence Name 27-K101282-0022-243-F08C-OriL
GenBank Accession LN498312 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K101282-0022-243-F08C-OriL
TCGGAATGAGCCTTTACATTGAGAGGTGAGATAGTGACCTTTGCTTCGATTGTCTCGATC
AACGTATCAACTCTGACTTCTCCGATCAGATCGTCTTCTCTTACGGTGTCTCCGATTCGC
CGCTACCTTTTGGATCCTCCGCCGTCGTTAAGGTGGATTTTTCAATTGTCATTATTTGAA
TTGTAGATTTCAATGGTGGAAGTATTGGAATTTAAGCTGCTTTAAATGGCGTTTGTGTAG
GTTTCTGATTCTAGCGAGGAATTTTCTGCTTCTTGTTCTTCTTGTGAATCCACTTCTTCT
CAGTTCATTTTGGAGTATCTCCGGAAGGACGAACATGGCTGCTTAGCTAAATACGTGTAC
GTTCTTGTTTATGTTCTCCTTAGGCTAACGATTTGATTCTATCAATCATTGTGGTTGAGC
AAATTATTCTTTTTCTGCAGCGACAAATTTGTTGTTAAGGATCGAGAGGGTAATTCAAAC
GATGCTGTTGAATCTGATGAGTGTTTGGATTGTTCAACCAGTGGCTCTCAAGCTACTGAA
GATGATGACACAGAGAATATTACTTGTGGAAGTGTTACTTGCGAGCATTCAGGTAGTTTT
T

Last Updated on 06.12.2018 16:11