Sequences from confirmation amplicon


Confirmation sequences for line 036A10 and hit At5g18640 are shown.
Line ID 036A10
Locus At5g18640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6215493
Primers used for PCR AY13 - 8474, show primer details
   
Sequence Name 43-K017552-022-036-A10C-AY13
GenBank Accession LN504671 [GenBank]
Sequencing primer gene specific primer, AY13: 5'-TACTTGACCCTCCCTACTCTAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K017552-022-036-A10C-AY13
AAACCCGGTATTGCTAATAGAAATATAGGATCGTTATTTACTACCTCGTTACACCCGAGC
CATCTGACTATTGGTCTGGGATTGTCACAACTCGTCTTATGTCGCGTACCGTCACTAGGA
CATTGGACATTTTTTGGAAGTATCCAGTATAATGGTTAGCTCTTGTTGTTTCAGATCGGT
AATGGGAAATAGCATATCTGACCATTTAACGTACTTTGGAGTGGAGTTGATGGGTGAGAC
TTGGAGGCAATGTAACATAGTGATGGGCCATGAGATGGAGAGTTACAGTAGGAAAGATTC
AAAGGGTAACATATTCCTTTCGCGGACAGTTCCTTCCACGGAGGTAATCAAAACAAAAAG
TATATCCAAAACCGGAATCTCAAGTCTATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGC
   
Sequence Name 44-K017552-022-036-A10C-8409
GenBank Accession LN505028 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K017552-022-036-A10C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATAGACTTGAGATTCCGGTTTT
GGATATACTTTTTGTTTTGATTACCTCCGTGGAAGGAACTGTCCGCGAAAGGAATATGTT
ACCCTTTGAATCTTTCCTACTGTAACTCTCCATCTCATGGCCCATCACTATGTTACATTG
CCTCCAAGTCTCACCCATCAACTCCACTCCAAAGTACGTTAAATGGTCAGATATGCTATT
TCCCATTACCGATCTGAAACAACAAGAGCTAACCATTATACTGGATACTTCCAAAAAATG
TCCAATGTCCTAGTGACGGTACGCGACATAAGACGAGTTGTGACAATCCCAGACCAATAG
TCAGATGGCTCGGGTGTAACGAGGTAGTAAATAACGATCCTATATTTCTATTAGCAATAC
CGGGTTTTTTCTTGCTAGAGTAGGGA

Last Updated on 06.12.2018 16:11