Sequences from confirmation amplicon


Confirmation sequences for line 359H03 and hit At5g19700 are shown.
Line ID 359H03
Locus At5g19700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6661048
Primers used for PCR 15po - 8474, show primer details
   
Sequence Name 11-K115208-0022-359-H03C-15po
GenBank Accession LN491244 [GenBank]
Sequencing primer gene specific primer, 15po: 5'-AAGGCTAGGTTATCAGCCATAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115208-0022-359-H03C-15po
GGGCGTAAGCGAAGTTCGGGGAAGGATCTTTCACAAACGACGTTGCTATCATCAAATTAA
CCGCAGCCGCGTTGCCGATTCTCGGGCTTTGCGAGCTCGGGAACTGCCCGCAAACAGTAG
GTTGCGGGGTTGTTAGAGGCACTGCACGGCCGTCCATGGCAGCGAACATAAACCTAGGAG
CGTTTTACCTCGTTGGAACGCCTGTAGCCGTTGGGCTAACGTTTTGGATGAACCATTTTA
GATGATTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCC
   
Sequence Name 04-K115207-0022-359-H03C-8409
GenBank Accession LN488035 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115207-0022-359-H03C-8409
AAAACGTTAGCCCAACGGCTACAGGCGTTCCAACGAGGTAAAACGCTCCTAGGTTTATGT
TCGCTGCCATGGACGGCCGTGCAGTGCCTCTAACAACCCCGCAACCTTACTGTTTGCGGG
CAGTTCCCGAGCTCGCAAAGCCCGAGAATCGGCAACGCGGCTGCGGTTAATTTGATGATA
GCAACGTCGTTTGTGAAGATCCATCCCCAGACATCGCTTACGCCCCAAGCAAACGCAGAC
GCCGTCAGACCCATTACCCCAGCAAAGGAAACCGCCACTATGGCTGATACCCTAGC

Last Updated on 06.12.2018 16:11