Sequences from confirmation amplicon


Confirmation sequences for line 329E02 and hit At5g20410 are shown.
Line ID 329E02
Locus At5g20410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6898062
Primers used for PCR AY15 - 8474, show primer details
   
Sequence Name 49-K017629-022-329-E02C-AY15
GenBank Accession LN506674 [GenBank]
Sequencing primer gene specific primer, AY15: 5'-TGGCAAAAGAAGAGGTAAGATTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K017629-022-329-E02C-AY15
GCTAGCAATCAAGACTCGTTGCTCTGAGAGCTCATCAATGTCTTTGACAATGTCGAAGAC
TGCCTCAGGCTGAGCTAGTTTACGTGCATTGTCTGAAGTTTGTTCCAACTCATCTGTCTT
TGTGCTAAACCATTCCCCAACGATTCTAGCTGTCTCTTTGGGACTTCTTGTGAATACTCC
TGCACCATTCTCCACTACATACGGCACATTCCCTTTCTCCTGTTTGGTTTCAAGTGTAAT
TGATATGAGAATTATACATATATATATATATGAGCAGGAAACATTAAAAATGCAGATCTC
AACCAACCTGTCCAGGAATGTAATCGTTGAGGATGATAGGAAGTGATCGAATCAGCGATT
CAGCGATTGTTCCTGGTCCAGCCTGCAAGATTTGTAGAACATTTAGCTTTATGAACATTT
TGGTTAACAGAGGTAGGCATAAAAACCAAATTCCTCTCACTTTTGTGATGATGCAGTCTA
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 53-K017629-022-329-E02C-8409
GenBank Accession LN507992 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K017629-022-329-E02C-8409
TAGATTTCCCAGACATGTAGACTGCATCATCACAAAAGTGAGAGGAATTTGGTTTTTATG
CCTACCTCTGTTAACCAAAATGTTCATAAAGCTAAATGTTCTACAAATCTTGCAGGCTGG
ACCAGGAACAATCGCTGAATCGCTGATTCGATCACTTCCTATCATCCTCAACGATTACAT
TCCTGGACAGGTTGGTTGAGATCTGCATTTTTAATGTTTCCTGCTCATATATATATATAT
GTATAATTCTCATATCAATTACACTTGAAACCAAACAGGAGAAAGGGAATGTGCCGTATG
TAGTGGAGAATGGTGCAGGAGTATTCACAAGAAGTCCCAAAGAGACAGCTAGAATCGTTG
GGGAATGGTTTAGCACAAAGACAGATGAGTTGGAACAAACTTCAGACAATGCACGTAAAC
TAGCTCAGCCTGAGGCAGTCTTCGACATTGTCAAAGACATTGATGAGCTCTCAGAGCAAC
GAGGTCCTCTTGCTAGCGTCTCTTACAATCTTACCTCTTCTTTTTG

Last Updated on 06.12.2018 16:11