Sequences from confirmation amplicon


Confirmation sequences for line 733D01 and hit At5g22330 are shown.
Line ID 733D01
Locus At5g22330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7391453
Primers used for PCR 02ja - 8474, show primer details
   
Sequence Name 36-K112831-0022-733-D01C-02ja
GenBank Accession LN502288 [GenBank]
Sequencing primer gene specific primer, 02ja: 5'-CCCATTAGACATTCTCCATATTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K112831-0022-733-D01C-02ja
ATGAGATGTTTCACAAAATGTGCGATTAGTGTTCTTCTCATGAGATGTATTTTTCTTGTT
GCTCATGCAAAAGCTTTGCTGAAGATTTAGCATCCAAGTAGAGTGATGTTACTTCCTCTA
TATCAGCCTGTCAAAATTTAAGATGAAGGATTAATAACATAACATCCACATATTTTACAT
TTGTTTTATTGTTCTAAGATTTCACAAGTAAATCTACCTTGCAAATATTGTCACGGCCAT
TCATTTTCGCTACAATGCTGGCAGGAGACAGAAGCTGCACAGCGTGCCTGAGAGTGAAAG
CACAAAAAAGGTTTTAGTATTTAGATTCACGAACAGAAATTTTGCTTAGAAAAGCAATGA
TGACAGTACCTTAGTGAAGTTCTTTGCCCAATCTCCCCAAGTAGAACCAAGCATTCTTCA
TCCACGGTTAATTCTTCAACTTGCGCACGAATGGCTATAATCTGTAAATGGATCCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCACGGTTATT
   
Sequence Name 07-K112829-0022-733-D01C-8409
GenBank Accession LN489350 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K112829-0022-733-D01C-8409
CATTTCATTGAAATATCCTGGATCCTTTACAGATTATAGCCATTCGTGCGCAAGTTGAAG
AATTAACCGTGGATGAAGAATGCTTGGTTCTACTTGGGGAGATTGGGCAAAGAACTTCAC
TAAGGTACTGTCATCATTGCTTTTCTAAGCAAAATTTCTGTTCGTGAATCTAAATACTAA
AACCTTTTTTGTGCTTTCACTCTCAGGCACGCTGTGCAGCTTCTGTCTCCTGCCAGCATT
GTAGCGAAAATGAATGGCCGTGACAATATTTGCAAGGTAGATTTACTTGTGAAATCTTAG
AACAATAAAACAAATGTAAAATATGTGGATGTTATGTTATTAATCCTTCATCTTAAATTT
TGACAGGCTGATATAGAGGAAGTAACATCACTCTACTTGGATGCTAAATCTTCAGCAAAG
CTTTTGCATGAGCAACAAGAAAAATACATCTCATGAGAAGAACACTAATCGCACATTTTG
TTGAAACATCTCATAATTTTTCTGTCGTTTTATTTTGTAATGTCGGAATATGGAGAATGT
CTAATGGG

Last Updated on 06.12.2018 16:11