Sequences from confirmation amplicon


Confirmation sequences for line 844B05 and hit At5g22360 are shown.
Line ID 844B05
Locus At5g22360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7404948
Primers used for PCR MN27 - 8474, show primer details
   
Sequence Name 47-K028160-022-844-B05C-8409
GenBank Accession LN506087 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K028160-022-844-B05C-8409
GATCCATGTAGATTTCCCGGACACGAAGCGAACATTGAGAAGATAATGGAAAGAGGTGAT
AGGATTGAGCTTCTTGTTGATAAAACAGCAACAATGCAAGATAGCTCGTTTCACTTCAGG
AAGCAATCTAAGCGCCTTCGCCGAGCTCTTTGGATGAAAAATGCTAAGCTCCTGTAAGGA
TCTCTCTCACTTCTAGATTCTCTTCATTCCTTTTCTTTGCTAACTAAGAGATACGCTCTT
CTACATTCTAGGTGTTTCAACTCGCCTTTGCAGCATAGTTTATTGATATGTTTCTTAAAC
TAGAGATACAGTAGTTCTAAGATTCAACATTATTTTCGATTTTGGGAAATTCTCCTCGAC
AAAATATCTAATTTTTGGCTGATAGGAACTACACTGCCTTGATTCCACCTCTGCTTAAAG
AAATTTCATTATTGTTTTGTTTTGATGCTTGAGTTCCAGATCACTTTTATTAATCAACAA
AACTGCTTTTCTTGTTTGTGTTCGGCAGGGTCTTGTTGACATGCTTGATAGTTTTCTTGC
TGTACATAATAATCGCATCTTTCTGCGGAGGAATCACTTTACCATCATGCAGATCTTAAA
ATCTGGCGGCCTTATCTAAGGTATACTGGAAA
   
Sequence Name 48-K028160-022-844-B05C-MN27
GenBank Accession LN506402 [GenBank]
Sequencing primer gene specific primer, MN27: 5'-GATTTACAAAAACAGTGGTCCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K028160-022-844-B05C-MN27
CAGTATACCTTAGATAAGGCCGCCAGATTTTAAGATCTGCATGATGGTAAAGTGATTCCT
CCGCAGAAAGATGCGATTATTATGTACAGCAAGAAAACTATCAAGCATGTCAACAAGACC
CTGCCGAACACAAACAAGAAAAGCAGTTTTGTTGATTAATAAAAGTGATCTGGAACTCAA
GCATCAAAACAAAACAATAATGAAATTTCTTTAAGCAGAGGTGGAATCAAGGCAGTGTAG
TTCCTATCAGCCAAAAATTAGATATTTTGTCGAGGAGAATTTCCCAAAATCGAAAATAAT
GTTGAATCTTAGAACTACTGTATCTCTAGTTTAAGAAACATATCAATAAACTATGCTGCA
AAGGCGAGTTGAAACACCTAGAATGTAGAAGAGCGTATCTCTTAGTTAGCAAAGAAAAGG
AATGAAGAGAATCTAGAAGTGAGAGAGATCCTTACAGGAGCTTAGCATTTTTCATCCAAA
GAGCTCGGCGAAGGCGCTTAGATTGCTTCCTGAAGTGAAACGAGCTATCTTGCATTGTTG
CTGTTTTATCAACAAGAAGCTCAATCCTATCACCTCTTTCCATTATCTTCTCAATGTTCG
CTTCGTGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATT

Last Updated on 06.12.2018 16:11