Sequences from confirmation amplicon


Confirmation sequences for line 298C10 and hit At5g22940 are shown.
Line ID 298C10
Locus At5g22940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7678320
Primers used for PCR MK62 - 8474, show primer details
   
Sequence Name 01-K101324-0022-298-C10C-8409
GenBank Accession LN486505 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K101324-0022-298-C10C-8409
TTCGGACTGAGCCTTTAAATTGAATATATCCTAAGAATATTAGCGGACGCTTTTTCTACA
TTTTTTTCTTGTTGACTTTGACTTTTGTGTTTTTGGATTTCTCGTTTAATTTACTAAAAT
GCCATTTTCTCCCGGCGTGTAGGGGAGTGAGAACGGCGATTCTAAAGAAATTCGGAGGAA
GAAGGCGGTTTTATCTAAACCGGCACCGGTTTGCTGGATACCGATCAGAAATCGTGAGAT
CAGTGTTTTGTCTCTGTCCTTTAGGGTGGGCACCATGGAGTCCTAGACTAGTGGAATCCG
CCGTGTTAGGTTGTGTTCCCGTTGTTATCGCCGATGGGATTCAATTACCGTTCTCGGAGA
CGGTACAATGGCCGGAAATCTCACTAACGGTGGCGGAGAAAGACGTGAGGAATCTACGAA
AGGTTTTGGAACACGTGGCGGCTACTAATTTGTCTGCAATACAAAGAAACTTACATGAAC
CGGTGTTTAAGCGGGCCCTCTTGTACAATGTGCCGATGAAAGAAGGAGACGCCACGTGGC
ATATTCTCGAGTCGTTGTGGAGGAAGCTCGATGATCGGTCGTACAGAAGGTCAAGGGTTT
TAAGCCAACGAGAAGTCGACATGTAGGATTCTGTGTCTG
   
Sequence Name 01-K101341-0022-298-C10C-MK62
GenBank Accession LN486506 [GenBank]
Sequencing primer gene specific primer, MK62: 5'-TAGCATCCATTCAGACACAGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K101341-0022-298-C10C-MK62
CCTTGACCTTCTNACGACCGATCATCGAGCTTCCTCCACAACGACTCGAGAATATGCCAC
GTGGCGTCTCCTTCTTTCATCGGCACATTGTACAAGAGGGCCCGCTTAAACACCGGTTCA
TGTAAGTTTCTTTGTATTGCAGACAAATTAGTAGCCGCCACGTGTTCCAAAACCTTTCGT
AGATTCCTCACGTCTTTCTCCGCCACCGTTAGTGAGATTTCCGGCCATTGTACCGTCTCC
GAGAACGGTAATTGAATCCCATCGGCGATAACAACGGGAACACAACCTAACACGGCGGAT
TCCACTAGTCTAGGACTCCATGGTGCCCACCCTAAAGGACAGAGACAAAACACTGATCTC
ACGATTTCTGATCGGTATCCAGCAAACCGGTGCCGGTTTAGATAAAACCGCCTTCTTCCT
CCGAATTTCTTTAGAATCGCCGTTCTCACTCCCCTACACGCCGGGAGAAAATGGCATTTT
AGTAAATTAAACGAGAAATCCAAAAACACAAAAGTCAAAGTCAACAAGAAAAAAATGTAG
AAAAAGCGTCCGCTAATATTCTTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11