Sequences from confirmation amplicon


Confirmation sequences for line 322E06 and hit At5g23000 are shown.
Line ID 322E06
Locus At5g23000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7696028
Primers used for PCR KT02 - 8474, show primer details
   
Sequence Name 02-K018063-022-322-E06C-KT02
GenBank Accession LN486829 [GenBank]
Sequencing primer gene specific primer, KT02: 5'-ACCTTAAACCACAACAAAGAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K018063-022-322-E06C-KT02
TCCAAAAGGTTATCTAGGATTAAGAACATGTGTATGAACTTACCGGCTTTGAGGGGGAAA
GAGATCCAATTTCCACCATTACCATACTTTTGAATGTAATCTCTAAGTTTAGAGTCTTCT
TCAGGCGACCAAGGCCCTCGCTTCACTTTTGTCTTGTCGCAACACGGAGCTCTTCCCATT
TCTCTCGTTAGTGAATTGAAGTTTGAAGAAGAGAAAAGAAAAAGAGAGAAATTGTTATCT
CTTACTTGCTTTTTTTAGGATGGACTTTTTGTGTTTATGGAGGAGTAGTTGAATATGTGC
CCTTTTAATAAGAGACTTGGATTTGGACACACCCTATCAATTATTATTATATATTATTAT
CTGGAGAACCAATTATTTATTTCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACG
   
Sequence Name 06-K018063-022-322-E06C-8409
GenBank Accession LN488595 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K018063-022-322-E06C-8409
CCGGAATGAAGCCATTTACAATTGAATATGAAATAAATAATTGGTTCTCCAGATAATAAT
ATATAATAATAATTGATAGGGTGTGTCCAAATCCAAGTCTCTTATTAAAAGGGCACATAT
TCAACTACTCCTCCATAAACACAAAAAGTCCATCCTAAAAAAAGCAAGTAAGAGATAACA
ATTTCTCTCTTTTTCTTTTCTCTTCTTCAAACTTCAATTCACTAACGAGAGAAATGGGAA
GAGCTCCGTGTTGCGACAAGACAAAAGTGAAGCGAGGGCCTTGGTCGCCTGAAGAAGACT
CTAAACTTAGAGATTACATTGAAAAGTATGGTAATGGTGGAAATTGGATCTCTTTCCCCC
TCAAAGCCGGTAAGTTCATACACATGTTCTTAATCCTAGATAACCTTTTCGAGCTTCTAA
GCCCAAATAAGAGCTTATGCCATTCTTTGTTGT

Last Updated on 06.12.2018 16:11