Sequences from confirmation amplicon


Confirmation sequences for line 121F07 and hit At5g23230 are shown.
Line ID 121F07
Locus At5g23230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7826265
Primers used for PCR NB42 - 8474, show primer details
   
Sequence Name 22-K101268-0022-121-F07C-8409
GenBank Accession LN496042 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K101268-0022-121-F07C-8409
TGACGAACTATGCTGTGAGACGACGGCGCGTGAAGCGTTTGTAAAGGGGTTTAGGGTTTT
TTTCTCGACGGACGCGACTGCGACGGTTAATGAAGAGCTTCACGAGGCTACTCTAATGAA
TCTCGCGTATGGCTTTGCTTATCTCGTCGATTGCGATAGACTCCGGCGAGGTCTACTCAG
TAGTTAATAAAAGATGACTACACCTTTTGTTATTTGTGTTTATTGTGTGTGTAAGAGCAT
CTGGTGATAAAAGTGGAATGTCTTTAGTCGTTAGATTAAAATTTTAATTGATAAACTGTA
AAAGTATTAAAACCAATAAGAAGGTGACATGTAGGTAGAAAATGTCTTTAAGAGATGTCT
TTGTGTATTTTTGGAGACACTATTCTTTTCTCTCTTCACAAATATCTTTAATTTATATTA
TAAAAATAATAAAGACACCCATTAATATGACTAATGGAGGTGCTTTAAGTGTAATTAAGT
AAGTAACAATAATACCGTTGTAAAATTTTCGTAGAAAAAATTGATAACTGCACCTACATT
TTTTACTGTCTAGACTGGTTACGAGTATTCATGACACTAGTACGTCCATGCATATTGCAT
   
Sequence Name 22-K101274-0022-121-F07C-NB42
GenBank Accession LN496043 [GenBank]
Sequencing primer gene specific primer, NB42: 5'-TTATGCAATATGCATGGACGTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K101274-0022-121-F07C-NB42
AATGTAGGTGCAGTTATCAATTTTTTCTCGAAAATTTTACAACGGTATTATTGTTACTTA
CTTAATTACACTTAAAGCACCTCCATTAGTCATATTAATGGGTGTCTTTATTATTTTTAT
AATATAAATTAAAGATATTTGTGAAGAGAGAAAAGAATAGTGTCTCCAAAAATACACAAA
GACATCTCTTAAAGACATTTTCTACCTACATGTCACCTTCTTATTGGTTTTAATACTTTT
ACAGTTTATCAATTAAAATTTTAATCTAACGACTAAAGACATTCCACTTTTATCACCAGA
TGCTCTTACACACACAATAAACACAAATAACAAAAGGTGTAGTCATCTTTTATTAACTAC
TGAGTAGACCTCGCCGGAGTCTATCGCAATCGACGAGATAAGCAAAGCCATACGCGAGAT
TCATTAGAGTAGCCTCGTGAAGCTCTTCATTAACCGTCGCAGTCGCGTCCGTCGAGAAAA
AAACCCTAAACCCCTTTACAAACGCTTCACGCGCCGTCGTCTCACAGCATAGGTTCGTCA
TCACTCCGATNACGATCACCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
NAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCA
Primers used for PCR NB43 - 3144, show primer details
   
Sequence Name 23-K101268-0022-121-F07C-3144
GenBank Accession LN496484 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101268-0022-121-F07C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAT
CACTGTACGTGCTCTTCTCCACGATTTCGTCTGGTCCGGTGACCTGGCGATTGATTTCCG
GGATGATTTCGGAATCAGTGGTTCCGTCGAGGATTAGATCGCCGTTCCACCACTCTCCGA
GCATGCCGTGGTCGGTTGGGGATTTGTGGTTGTGGCGCGTGAAGAATACGGGGATTGAAG
CGCGTCGGCNGATGTCGATGGTGGTGAGAGCGTTTTGGAGGATTGGTTCAGCCATAGAGT
AGAAGTGATTCTGCATATCGATGACGAGAAGAGCTGCGATTTTAGGATTTGGCTCTCGCT
TTCGTGTCTCGTACGTTCTCGATGATGAAGAAGCCATTTCTGGCGGTGAAGATGAATCTC
TTGATCGGTGGAGATATATTTGGGAAACGAAA
   
Sequence Name 23-K101274-0022-121-F07C-NB43
GenBank Accession LN496485 [GenBank]
Sequencing primer gene specific primer, NB43: 5'-CTTTCGTTCCCAAATATATCTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101274-0022-121-F07C-NB43
GCCAGAATGGCTTCTTCATCATCGAGAACGTACGAGACACGAAAGCGAGAGCCAAATCCT
AAAATCGCAGCTCTTCTCGTCATCGATATGCAGAATCACTTCTACTCTATGGCTGAACCA
ATCCTCCAAAACGCTCTCACCACCATCGACATCTGCCGACGCGCTTCAATCCCCGTATTC
TTCACGCGCCACAACCACAAATCCCCAACCGACCACGGCATGCTCGGAGAGTGGTGGAAC
GGCGATCTAATCCTCGACGGAACCACTGATTCCGAAATCATCCCGGAAATCAATCGCCAG
GTCACCGGACCAGACGAAATCGTGGAGAAGAGCACGTACAGTGATTCAAACACTGATAGT
TTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAA
ATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCC
TTACGTCAGTGGAGATAT

Last Updated on 06.12.2018 16:11