Sequences from confirmation amplicon


Confirmation sequences for line 421A10 and hit At5g23660 are shown.
Line ID 421A10
Locus At5g23660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7971883
Primers used for PCR NA34 - 8474, show primer details
   
Sequence Name 36-K101564-0022-421-A10C-8409
GenBank Accession LN502162 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K101564-0022-421-A10C-8409
CATTTACATTGAAGCCAAAGTCAAAATAGTCAACAAATAGGTTTCTTATTAGGGTTTCTT
GGGCAAACTCAAGTAGTTGCAGCACTGTTTCTAACTCCCTGACCATTTTCAATCTCAGCA
TTTCGATCGTTACAGTTACATGTGTTCACCGATCGGACGACGGTGATCGCTACTGGTTCA
GGAGATGTGAGTGTACCTAACTTCACCATATCGATGCTCACTTCTGGCAATTTCGCAGCC
TCAAGTTCTTTCTCAACCAAATCCGACGGCGTTTTGCAGTATTTGTAAACCACATAGAGT
ATCATTTGAAGTGCACCTAGAACAAACCCAATCACGTTTGGGAACTGCATAAATTGAAAG
ACATATAAAAAGGTGAAATTAAAGATAATTTATTAGTGCTCANAGGCCAA
   
Sequence Name 36-K101577-0022-421-A10C-NA34
GenBank Accession LN502163 [GenBank]
Sequencing primer gene specific primer, NA34: 5'-TTTGGCCTTTGAGCACTAATAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K101577-0022-421-A10C-NA34
ATCCTTTTATTGTCTTTCATTTTGCAGTTCCCAAACGTGATTGGGTTTGTTCTAGGTGCA
CTTCAAATGATACTCTATGTGGTTTACAAATACTGCAAAACGCCGTCGGATTTGGTTGAG
AAAGAACTTGAGGCTGCGAAATTGCCAGAAGTGAGCATCGATATGGTGAAGTTAGGTACA
CTCACATCTCCTGAACCAGTAGCGATCACCGTCGTCCGATCGGTGAACACATGTAACTGT
AACGATCGAAATGCTGAGATTGAAAATGGTCAGGGAGTTAGAAACAGTGCTGCAACTACT
TGAGTTTGCCCAAGAAACCCTAATAAGAAACCTATTTGTTGACTATTTTGACTTTGGCTT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATNTC
CGCACGGTTATT

Last Updated on 06.12.2018 16:11