Sequences from confirmation amplicon


Confirmation sequences for line 704D05 and hit At5g26660 are shown.
Line ID 704D05
Locus At5g26660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9332949
Primers used for PCR DM26 - 8474, show primer details
   
Sequence Name 31-K101829-0022-704-D05C-8409
GenBank Accession LN500102 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101829-0022-704-D05C-8409
CATTTACATTGAATATGTCTAGTGATGTAATTGAGAAGTTTCTCATCTTCTTCAGGAGAC
CAAAGGCCTTTTCTTAGCTTCTGCTTAAAACAACAAGAATGTCTTCCCATTTTTTATAAA
AGTAATGAAGAAAGTATAGATTGTTGCAGAGAGAGTTTTATGGTTCTTGGAGGTTCTATA
AGCTTGAAGAGGAAGGAAGAAGATGGAGAAAATTATTATAAAATGAGAGAAACTATGATT
GGCGATATTCTGGTGTAAACACTTTTGTTTTATAATCGTTGGGTGAATTAATTGATACCT
TTTATGGGACCACCTTATTC
   
Sequence Name 75-K101832-0022-704-D05C-DM26
GenBank Accession LN513214 [GenBank]
Sequencing primer gene specific primer, DM26: 5'-GAATAAGGTGGTCCCATAAAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K101832-0022-704-D05C-DM26
CAGAAATATCGCCAATCATAGTTTCTCTCATTTTAAATAATTTTCTCCATCTTCTTCCTT
CCTCTTCAAGCTTATAGAACCTCCAAGAACCATAAAACTCTCTCTGCAACAATCTATACT
TTCTTCATTACTTTTATAAAAAATGGGAAGACATTCTTGTTGTTTTAAGCAGAAGCTAAG
AAAAGGCCTTTGGTCTCCTGAAGAAGATGAGAAACTTCTCAATTACATCACTAGACATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
C

Last Updated on 06.12.2018 16:11