Sequences from confirmation amplicon


Confirmation sequences for line 214E12 and hit At5g33280 are shown.
Line ID 214E12
Locus At5g33280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:12551614
Primers used for PCR BL98 - 8474, show primer details
   
Sequence Name 31-K105305-0022-214-E12C-BL98
GenBank Accession LN500160 [GenBank]
Sequencing primer gene specific primer, BL98: 5'-ATGAACCAAGAAACCAAAGAGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K105305-0022-214-E12C-BL98
TTGGTGGTTCGAGATTCGAGTTTTGTTATTGATTCCCTTAACAAACATACAAAACTCAAG
AGACTCTACTGTGTTCACCATTTTACTTATTATTCATCTATAGGTATCAAGTATCACACG
TACTAGTGCCTACCATAATCTAATAAGGACCAGGGAGAAAGAAGAAACCAAAATCACTTA
CATTGGAGGTTTTTGGTATAACCAGTAAGTGCCTTATGCCAACCTCACGGAAGAGAATAA
GGGCCTTTGCAAGTGACATTGTCTCCACAACAGTGTACGGAGAGGCATTAGAGAATGGGT
GCAAATCCAAATACATATTCAATTCTTCCTCGCTTAATTCCACATCTTCTATCTTATCAC
TCCTCCCAGAACCCTTCTTAGCAAACTCCTCTGCCTTGAATTGGGAAAGGGTATTGGAGT
CACAAGCTACTGGACTAGGCATGAATACTCGTTTCTTTAACAGAGTCAGAATATGAGCCC
GGAGGATTAGACCGTGTAGAACAGGAGCTGCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 15-K105306-0022-214-E12C-8409
GenBank Accession LN492967 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K105306-0022-214-E12C-8409
TCCGGGCTCTATTCTGACTCTGTTAAAGAAACGAGTATTCATGCCTAGTCCAGTAGCTTG
TGACTCCAATACCCTTTCCCAATTCAAGGCAGAGGAGTTTGCTAAGAAGGGTTCTGGGAG
GAGTGATAAGATAGAAGATGTGGAATTAAGCGAGGAAGAATTGAATATGTATTTGGATTT
GCACCCATTCTCTAATGCCTCTCCGTACACTGTTGTGGAGACAATGTCACTTGCAAAGGC
CCTTATTCTCTTCCGTGAGGTTGGCATAAGGCACTTACTGGTTATACCAAAAACCTCCAA
TGTAAGTGATTTTGGTTTCTTCTTTCTCCCTGGTCCTTATTAGATTATGGTAGGCACTAG
TACGTGTGATACTTGATACCTATAGATGAATAATAAGTAAAATGGTGAACACAGTAGAGT
CTCTTGAGTTTTGTATGTTTGTTAAGGGAATCAATAACAAAACTCGAAATCTCGAACCAC
CAATTAGTTCTCTATATATCTGGTAGCTCTTTGGTTTTNTGGTTC

Last Updated on 06.12.2018 16:11