Sequences from confirmation amplicon


Confirmation sequences for line 144B01 and hit At5g34850 are shown.
Line ID 144B01
Locus At5g34850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:13111064
Primers used for PCR KS78 - 8474, show primer details
   
Sequence Name 31-K101563-0022-144-B01C-8409
GenBank Accession LN500098 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101563-0022-144-B01C-8409
CATTTACATTGAATATTCCATATAGCCTTTTGGGACTTTGAAAACATGATGATCAAGTGG
TATATCAACCGCAGGCCATTCAGATCGAATAAAACTACTTGTGATTCCAGATTCTCCTCT
ATATAACAACAATACTGAGCTCAAGAACACAGAGATTATCACCAAATGATTCATCACGTC
ACCAAATCTCGACTCAGTTTTTTTACCTGTAGCCACAAAGAATCGCGAATCTGAATGTTG
ATTTCAAATCTAGAATGAAGCAAATCTTAAGATACAATCTGAGAAATTCGTTGTGATTTT
AAGATCTGAAGAAATCGAGAGAGAGGTTATGGATATTACCTGAGAGATGAAGGAGATGAC
ATTTGGGAGATTATCGTCTCGATGACATTAATAAGCCCGTCGGATATGTATGAATGAGCT
ATCGAAGAAGGTGACCACATACGTGGAGATTTATGATTGGTTTGAGGGAA
   
Sequence Name 31-K101576-0022-144-B01C-KS78
GenBank Accession LN500099 [GenBank]
Sequencing primer gene specific primer, KS78: 5'-TTCCCTCAACCAATCATAAATCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101576-0022-144-B01C-KS78
GCTATTCATACATATCCGACGGGCTTATTAATGTCATCGAGACGATAATCTCCCAAATGT
CATCTCCTTCATCTCTCAGGTAATATCCATAACCTCTCTCTCGATTTCTTCAGATCTTAA
AATCACAACGAATTTCTCAGATTGTATCTTAAGATTTGCTTCATTCTAGATTTGAAATCA
ACATTCAGATTCGCGATTCTTTGTGGCTACAGGTAAAAAAACTGAGTCGAGATTTGGTGA
CGTGATGAATCATTTGGTGATAATCTCTGTGTTCTTGAGCTCAGTATTGTTGTTATATAG
AGGAGAATCTGGAATCACAAGTAGTTTTATTCGATCTGAATGGCCTGCGGTTGATATACC
ACTTGATCATCATGTTTTCAAAGTCCCAAAAGGCTATATGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
AT

Last Updated on 06.12.2018 16:11