Sequences from confirmation amplicon


Confirmation sequences for line 569A11 and hit At5g35111 are shown.
Line ID 569A11
Locus At5g35111 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:13366693
Primers used for PCR 05vm - 8474, show primer details
   
Sequence Name 15-K118135-0022-569-A11C-05vm
GenBank Accession LN493170 [GenBank]
Sequencing primer gene specific primer, 05vm: 5'-CGCTTTTGGTAGTCGTTTTGTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118135-0022-569-A11C-05vm
ACTCATGTGACTTTGACTTCAAGTGACTTCCTTTTGGATCTCTCAAACTTAAAAAAAAGG
ACCAAAATGACTTATATATCAAACTCTGCAAAAAAATTAACCTTGTCAGCATAACATTTT
TTTCTTTCACCTCTTTTGTTTTCCTTGCATTTCAAGAAACTTAGTATAATGGTTATTTTG
CTTCTTTACTTCTAAACGTTTTCATACTTACATATAGGGATGTGACGATTCGATTCTGCT
AGACGATACGTCAAGCTTCAGAGGAGAACAAAACGCGGCCCCAAACCGCAATTCAGCTCG
TGGATTTAATGTGATCACTGATCAATAACATCAAATCAGCGTTGAGAAAGCATATTTCCC
GGATTGTATCTTGCGTTAACATCTAAGCCATTGCAGCTAGAGACTCCGTCGTCTTCTCTT
AGTCTTGTTTTGAAACTTAAGAAAATTAAACCGGTCTAACATATGAATTCGTCTTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
GGTT
   
Sequence Name 07-K118134-0022-569-A11C-8409
GenBank Accession LN489466 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118134-0022-569-A11C-8409
GCATTTCATTGAAGACGAATTCATATGTTAGACCGGTTTAATTTTCTTAAGTTTCAAAAC
AAGACTAAGAGAAGACGACGGAGTCTCTAGCTGCAATGGCTTAGATGTTAACGCAAGATA
CAATCCGGGAAATATGCTTTCTCAACGCTGATTTGATGTTATTGATCAGTGATCACATTA
AATCCACGAGCTGAATTGCGGTTTGGGGCCGCGTTTTGTTCTCCTCTGAAGCTTGACGTA
TCGTCTAGCAGAATCGAATCGTCACATCCCTATATGTAAGTATGAAAACGTTTAGAAGTA
AAGAAGCAAAATAACCATTATACTAAGTTTCTTGAAATGCAAGGAAAACAAAAGAGGTGA
AAGAAAAAAATGTTATGCTGACAAGGTTAATTTTTTTGCAGAGTTTGATATATAAGTCAT
TTTGGTCCTTTTTTTTAAGTTTGAGAGATCCAAAAGGAAGTCACTTTGAAGTCAAAGTCA
CATGAGTTTTTTCTAGAGTTACCGAATTACATCTTCTGAAGTTTATATACTACAAAACGA
CACCCAAAAGCG

Last Updated on 06.12.2018 16:11