Sequences from confirmation amplicon


Confirmation sequences for line 438E03 and hit At5g37800 are shown.
Line ID 438E03
Locus At5g37800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15037801
Primers used for PCR 10nz - 8474, show primer details
   
Sequence Name 11-K114835-0022-438-E03C-10nz
GenBank Accession LN491231 [GenBank]
Sequencing primer gene specific primer, 10nz: 5'-TTAACGAACAAAAGTGCAGAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K114835-0022-438-E03C-10nz
AAATAAATTTAAAAGGGTTTTAAGGAGGATTTAAGTTTTAATTTCTAATGAAATAGTTGA
TTTTTTCGTTGCCTTACTGCATCGTATGTAGAAATATAGACATTCATAATAAATATTTTT
TGTCTTTTAATTACTAAGAGGTTCGGATCTAGGTCTGTTTCTCGGGTAGGAAACCATAAC
ATTTAATATAGTTTCCCCTTAAATGTTATTCGTATCAGTGACTTCTAAACTTTGCATAGA
TGTATTATCGATTAAGCTATTAACACTTAATTACATGCATAATATCTCAAAACTTAACAA
CACTTAGTTCAATTCATGAAAACATTTTATAAAAGATATCTTAAAGTAAAACTCAGAAAC
CAAACTTTTGCAACATCACATTACGCATACTTGGAGAATTACAAAACATACAAAAATAGA
AACATACCTCCACATATATNACTATTACATATCTNATCTACTATAAGATTCCATGCTCGT
GTGGACCGACGTCTGACATATCCNGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 03-K114834-0022-438-E03C-8409
GenBank Accession LN487599 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114834-0022-438-E03C-8409
CTTATAGTAGATTAGATATGTAATAGTTATATATGTGGAGGTATGTTTCTATTTTTGTAT
GTTTTGTAATTCTCCAAGTATGCGTAATGTGATGTTGCAAAAGTTTGGTTTCTGAGTTTT
ACTTTAAGATATCTTTTATAAAATGTTTTCATGAATTGAACTAAGTGTTGTTAAGTTTTG
AGATATTATGCATGTAATTAAGTGTTAATAGCTTAATCGATAATACATCTATGCAAAGTT
TAGAAGTCACTGATACGAATAACATTTAAGGGGAAACTATATTAAATGTTATGGTTTCCT
ACCCGAGAAACAGACCTAGATCCGAACCTCTTAGTAATTAAAAGACAAAAAATATTTATT
ATGAATGTCTATATTTCTACATACGATGCAGTAAGGCAACGAAAAAATCAACTATTTCAT
TAGAAATTAAAACTTAAATCCTCCTTAAAACCCTTTTAAATTTATTTTTTCAGTTGTGAC
TGTTTAGCTTACGTCCATTTCTGCACTTTTGTTCGTTA

Last Updated on 06.12.2018 16:11