Sequences from confirmation amplicon


Confirmation sequences for line 682C07 and hit At5g37800 are shown.
Line ID 682C07
Locus At5g37800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15036033
Primers used for PCR LD07 - 8474, show primer details
   
Sequence Name 10-K027155-022-682-C07C-8409
GenBank Accession LN490502 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K027155-022-682-C07C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGATAACATAACTACA
CAAACATTGTCTCTCTTTGCTTACCAATCTACAATCTTAAAACCATTTTTCGTTGTTTCG
ATTTCTCAAATCTCCTTAACCTCTCAAAGCAAAACCAAAGACATAAAAAAGAAACCCCAT
ACATCTCACTAGAACACCCTTTAGTACCAATGTCACTCATTAACGAACATTGCAATGAGC
GTAATTACATCTCAACCCCAAATTCTTCAGAAGATCTCTCTTCACCACAGAATTGCGGAT
TAGACGAAGGAGCTTCAGCTTCAAGCAGTAGCACCATAAATTCTGATCATCAAAATAATC
AAGGGTTTGTGTTTTACCCTTCCGGGGAAACCATTGAAGATCATAATTCTTTGATGGATT
TCAATGCTTC
   
Sequence Name 14-K027155-022-682-C07C-LD07
GenBank Accession LN492353 [GenBank]
Sequencing primer gene specific primer, LD07: 5'-GTGAAGAATGATGAAGCATTGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K027155-022-682-C07C-LD07
CATCAAAGAATTATGATCTTCAATGGTTTCCCCGGAAGGGTAAAACACAAACCCTTGATT
ATTTTGATGATCAGAATTTATGGTGCTACTGCTTGAAGCTGAAGCTCCTTCGTCTAATCC
GCAATTCTGTGGTGAAGAGAGATCTTCTGAAGAATTTGGGGTTGAGATGTAATTACGCTC
ATTGCAATGTTCGTTAATGAGTGACATTGGTACTAAAGGGTGTTCTAGTGAGATGTATGG
GGTTTCTTTTTTATGTCTTTGGTTTTGCTTTGAGAGGTTAAGGAGATTTGAGAAATCGAA
ACAACGAAAAATGGTTTTAAGATTGTAGATTGGTAAGCAAAGAGAGACAATGTTTGTGTA
GTTATGTTATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11