Sequences from confirmation amplicon


Confirmation sequences for line 254D05 and hit At5g38410 are shown.
Line ID 254D05
Locus At5g38410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15377449
Primers used for PCR 57lx - 8474, show primer details
   
Sequence Name 18-K114874-0022-254-D05C-57lx
GenBank Accession LN494420 [GenBank]
Sequencing primer gene specific primer, 57lx: 5'-CTTTTGTCATGTGCAATAAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K114874-0022-254-D05C-57lx
GATTAAGGTGTGGCCCCAATTGGAAAGAAGAAGTTTGAGACTCTATCTTACCTCCCTGAC
CTTAGTGACGTCGAATTGGCTAAGGAAGTTGACTACCTTCTCCGCAACAAGTGGATTCCT
TGTGTTGAATTCGAGTTAGAGGTAATAAACACAAAAGTTTTCTTTTGTCTACTAATCATT
ATTATTTATTCGTTTTCATTGCTAGAAATATTAGCCTATAACCGGATTTTGTAGAACCGA
AATAGACTATATCACCTTGTGCATATCCTCCAATATCAATTGTATTGAATGGTTTTCTTA
TGTGTTTATAGCACGGATTTGTGTACCGTGAGCACGGAAACACTCCCGGATACTACGATG
GACGGTACTGGACAATGTGGAAGCTTCCATTGTTCGGATGCACCGACTCCGCTCAAGTGT
TGAAGGAAGTTGAAGAATGCAAGAAGGAGTACCCGGGCGCCTTCATTAGGATCATCGGAT
TCGACAACACCCGTCAAGTCCAATGCATCAGTTTCATTGCCTACAAGCCCCCAAGCTTCA
CCGAAGCTTAATTTCTTTTCTAAAACATTCTTATGAATTATCTCTGCTCATTTCATTTCC
TATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGC
   
Sequence Name 12-K114873-0022-254-D05C-8409
GenBank Accession LN491695 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114873-0022-254-D05C-8409
ATTGAATATATCATAGGAAATGAAATGAGCAGAGATAATTCATAAGAATGTTTTAGAAAA
GAAATTAAGCTTCGGTGAAGCTTGGGGGCTTGTAGGCAATGAAACTGATGCATTGGACTT
GACGGGTGTTGTCGAATCCGATGATCCTAATGAAGGCGCCCGGGTACTCCTTCTTGCATT
CTTCAACTTCCTTCAACACTTGAGCGGAGTCGGTGCATCCGAACAATGGAAGCTTCCACA
TTGTCCAGTACCGTCCATCGTAGTATCCGGGAGTGTTTCCGTGCTCACGGTACACAAATC
CGTGCTATAAACACATAAGAAAACCATTCAATACAATTGATATTGGAGGATATGCACAAG
GTGATATAGTCTATTTCGGTTCTACAAAATCCGGTTATAGGCTAATATTTCTAGCAATGA
AAACGAATAAATAATAATGATTAGTAGACAAAAGAAAACTTTTGTGTTTATTACCTCTAA
CTCGAATTCAACACAAGGAATCCACTTGTTGCGGAGAAGGTAGTCAACTTCCTTAGCCAA
TTCGACGTCACTAAGGTCAGGGAGGTAAGATAGAGTCTCAAACTTCTTCTTTCCAATTGG
TGGCCACACCTATNATCCCCAAATCCAATTCCAAATTAATATATCCNATGTTTTGTTTTA
TTGCA

Last Updated on 06.12.2018 16:11