Sequences from confirmation amplicon


Confirmation sequences for line 830D10 and hit At5g38565 are shown.
Line ID 830D10
Locus At5g38565 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15445400
Primers used for PCR 04up - 8474, show primer details
   
Sequence Name 56-K117939-0022-830-D10C-04up
GenBank Accession LN509227 [GenBank]
Sequencing primer gene specific primer, 04up: 5'-CCAACTGGAATTCACAGGTTTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K117939-0022-830-D10C-04up
ATTCACATTACAGCTCTCTGTAGGGTTTTGTCATGCTTGTGTTCTGTGTTTTCTCTTGTT
ACTAATTCGTTTGCTTGGCCTGAAAAAAAATCATTTGATGGTGTTTTTTTCTAGTGTTTT
ACAGTAACTTAGAATCTGTAAGAGTTTGACAAAAAAAATAAAAAACCTGTAAGATATGGA
CATATTCAATGGATTGCCAGATGATGTGCTGGTGAAGATTTTATCGTTTGTTCCGACAAA
AGTTGCTGTATCCACTTCCATTTTGTCTAAGCGTTGGGAGTTTCTTTGGATGTGGTTGCC
TAGACTCGATTTCGGTTCTCCAAAGACTGATCTTCTGGCTTTTCTCAACACTTGTCAATA
TGATAAGGAGGAGGAGGTTGGACTTGTGGATTTTATTGACAAGAAGCTGCCACTACACAG
AGCTCCGGTCTTAGAAAGCTTGCGACTCAATTTTTATCTTTATCAGTATAACACACATAT
TGAACCTGAATATATCGAACGGTGGATTGAAATTGCAGTTTCTCGCCATGTACGTGTGCT
GGAAATTTCTTATGAATGTTGGGATGAAAACATATTTCCATGTACGTCACCTTGAAACTC
GACAACGTGGTTCTCATTGATGTTCCCTCTACTGTTTCTTTTCCCTCTCTGAAAACTTTG
CAACTTGACAGTGTGACATTTGTAGAAGGAAAATCTCTTCAGGACATTCTATCTATTTGT
CCTGTTCTTGATGATCTATCGGTGATATGTTCTGTGCATCAAGATGTTAAAGAGTTCACT
ATTATCGTCCCATCTCTGCAGAGTTTAACTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTCAAAAATGT
   
Sequence Name 47-K117938-0022-830-D10C-8409
GenBank Accession LN506325 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117938-0022-830-D10C-8409
ATAATAGTGAACTCTTTACATCTTGATGCACAGAACATATCACCCATAGATCATCAAGAA
CAGGACAAATAGATAGAATGTCCTGAAGAGATTTTCCTTCTACAAATGTCACACTGTCAA
GTTGCAAAGTTTTCAGAGAGGGAAAAGAAACAGTAGAGGGAACATCAATGAGAACCACGT
TGTCGAGTTTCAAGGTGACGTACATGGAAATATGTTTTCATCCCAACATTCATAAGAAAT
TTCCAGCACACGTACATGGCGAGAAACTGCAATTTCAATCCACCGTTCGATATATTCAGG
TTCAATATGTGTGTTATACTGATAAAGATAAAAATTGAGTCGCAAGCTTTCTAAGACCGG
AGCTCTGTGTAGTGGCAGCTTCTTGTCAATAAAATCCACAAGTCCAACCTCCTCCTCCTT
ATCATATTGACAAGTGTTGAGAAAAGCCAGAAGATCAGTCTTTGGAGAACCGAAATCGAG
TCTAGGCAACCACATCCAAAGAAACTCCCAACGCTTAGACAAAATGGAAGTGGATACAGC
AACTTTTGTCGGAACAAACGATAAAATCTTCACCAGCACATCATCTGGCAATCCATTGAA
TATGTCCATATCTTACAGGTTTTTTATTTTTTTTGTCAAACTCTTACAGATTCTAAGTTA
CTGTAAAACACTAGAAAAAAACACCATCAAATGATTTTTTTTCAGGCCAAGCAAACGAAT
TAGTAACAAGAGAAAACACAGAACACAAGCATGACAAAACCCTACAGAGAGCTGTAATGT
TGAAATCTGAAACAGGACAAAACCCTGGAAAAAATTAAACCTG

Last Updated on 06.12.2018 16:11