Sequences from confirmation amplicon


Confirmation sequences for line 719B12 and hit At5g38670 are shown.
Line ID 719B12
Locus At5g38670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15476936
Primers used for PCR 20sf - 8474, show primer details
   
Sequence Name 32-K116390-0022-719-B12C-20sf
GenBank Accession LN500725 [GenBank]
Sequencing primer gene specific primer, 20sf: 5'-AATACACAAACTTGGGATCCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K116390-0022-719-B12C-20sf
GACTTTCACTAAAAATCGTAAGGTTTGACGGAAATTGCACCTGGTGAGCTACAAAGGTGT
GGATGCTTACAATTCCAAGGAAGGTAGATGGGACCTGGTTACACCAAGCATAGAACATAA
TAAGTATTTATATGATTGTTATCGCAATATAGGGAATGTTTGGTACACTATTGTAAAAGG
AGATATCTCAACGTCCGTATGGTGGTATCACAGTGAGGAAAGAGAGTGGAGAGACTTGAA
GGGTATGGTAGGATTACCTAAGTTCCCTATTGATGCTTGTCTTAGAATGGTTGATTATGG
TGGGAAGATGGCAGTTTTGTGGGACGACTATTTGCCTGGGCGCCGAAAGAACATGATTTG
GTGTGCGGACATTGCGCTTGAAATACGCGGAATTTTGGAAATATGGGGAAAAGTAGAATG
GTTTGCTCATGTGCTTGCAGTCCCTATACCATATGAATTCATCACGGTTCTAGCTGCTCC
TCTTTGATGAAAGCCTTGCTATCCTTGAGACTATTATTTTGTCCCTGGATTCGCTTCCAA
CTAATTTATGTCTAATATTTAGGGGCCTTGAACATTGTTATCAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCGATGAGTATGAATGGTCAAAATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 07-K116389-0022-719-B12C-8409
GenBank Accession LN489432 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K116389-0022-719-B12C-8409
TTACATTGAAATATCCTGAAAACAATCTTCAAGGCCCCTAAATATTAGACATAAATTAAT
TAGAAGTGAAACAAGGGACAAAATCATAGTTTCAAGGCTCGTAACGCATTCATCAAAGAG
TAGCAGCAAGAACCTTGACGAATTCATATTGTCTAGGGACTGTAAGCACATGAGCAAACC
ATTCAACTTTTCCCCATATTTCCAAACTTCCGCGTCTTTCAAGCGCAATCTCCGCACACC
AAATCATCTTCTTTCGGCGCCCAGGCAAATAGTCGTCCCACAAAACTGCCATCTTCCCAC
CATAATCAACCATTCTAAGACAAGCATCAATAGGGAACTTAGGTAATCCTACCATACCCT
TCAAGTCTCTCCACTCTCTTTCCTCACTGTGATACCACCATACGGACGTTGAGATATCTC
CTTTTACAATAGTGTACCAAACATTCCCTATATTGCGATAACAATCATATAAATACTTAT
TATGTTCTATGCTTGGTGTAACCAGGTCCCATCTACCTTCCTTGGAATTGTAAGCATCCA
CACCTTTGTAGCTCACCAGGTGCAATTTTCCGTCAAACCTTACGATTTTGTAGTGAAAGT
CGTGTTTTGTCTCGCTGCAAGGGACAGACAAAGGATCCC

Last Updated on 06.12.2018 16:11