Sequences from confirmation amplicon


Confirmation sequences for line 866H06 and hit At5g40990 are shown.
Line ID 866H06
Locus At5g40990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16419818
Primers used for PCR 18ih - 8474, show primer details
   
Sequence Name 68-K112758-0022-866-H06C-18ih
GenBank Accession LN512046 [GenBank]
Sequencing primer gene specific primer, 18ih: 5'-CACTGCAAGGTGATAAATTTGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K112758-0022-866-H06C-18ih
TTGAGAATGTTAGGTCTAAGTTAGGAGATGCTGAGGGGAAGAGGGTTATCTCAAGAGCTG
TTTATTTGTTTCATATTGGACTCAACGACTATCAATATCCATTCACTACAAATTCTTCCT
TATTTCAATCCATTTCCAACGAGAAATACGTAGATTATGTTGTCGGTAACATGACAGACG
TTTTCAAGGTAAAAAATTTTCAAAAGGGTTTTTGTAGAGTTTCTATTGTGAACAACTTTT
GGTCTGAAAAATGAGAGATGATGTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 28-K112757-0022-866-H06C-8409
GenBank Accession LN498918 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K112757-0022-866-H06C-8409
GCATTTCATTGAATATACATCATCTCTCATTTTTCGACCAAAAGTTGTTCACAATAGAAA
CTCTACAAAAACCCTTTTGAAAATTTTTTACCTTGAAAACGTCTGTCATGTTACCGACAA
CATAATCTACGTATTTCTCGTTGGAAATGGATTGAAATAAGGAAGAATTTGTAGTGAATG
GATATTGATAGTCGTTGAGTCCAATATGAAACAAATAAACAGCTCTTGAGATAACCCTCT
TCCCCTCAGCATCTCCTAACTTAGACCTTAACATTTCTTCAACCTTTTTGAAGTTGTTTA
ATTGTGTTCTCAAATTTATCACNTTGCAGTG

Last Updated on 06.12.2018 16:11